Roziasyahira, Mutazah and Hazrulrizawati, Hamid and Aizi Nor Mazila, Ramli and Mohd F. F., Mohd Aluwi and M. M., Yusoff (2020) In vitro cytotoxicity of Clinacanthus nutans fractions on breast cancer cells and molecular docking study of sulphur containing compounds against caspase-3. Food and Chemical Toxicology, 135 (110869). pp. 1-9. ISSN 0278-6915. (Published)
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Abstract
Clinacanthus nutans has attracted Malaysian public interest due to its high medicinal value in the prevention of cancer. Currently, the specific compound or compounds giving rise to the anticancer potential of C. nutans has not been investigated thoroughly. The extraction was carried out by MeOH at room temperature using the powdered bark of C. nutans, while chromatography was carried out on a silica gel RP-18 column using the crude methanolic extract. Six fractions collected from column chromatography were evaluated by MTT assay against two breast cancer cell lines: MDA-MB-231 and MCF-7. Amongst the fractions, A12 and A17 were shown to exhibit the highest activity. Two sulphur-containing compounds, viz., entadamide C (1) and clinamide D (2), were isolated from these fractions. Molecular docking simulation studies revealed that entadamide C and clinamide D could bind favourably to the caspase-3 binding site with the binding energy of −4.28 kcal/mol and −4.84 kcal/mol, respectively. This study provides empirical evidence for the presence of sulphur-containing compounds in the leaves of C. nutans that displayed anticancer effects which explains its ethnomedicinal application against breast cancer. The docking simulation study showed that both compounds could serve as important templates for future drug design and development.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Clinacanthus nutans; Cytotoxicity; MDA-MB-231; MCF-7; Sulphur containing compoun ds; Molecular docking; Caspase-3 |
| Subjects: | Q Science > Q Science (General) |
| Faculty/Division: | Faculty of Industrial Sciences And Technology Institute of Postgraduate Studies |
| Depositing User: | Dr. Hazrulrizawati Abd Hamid |
| Date Deposited: | 12 Feb 2020 07:12 |
| Last Modified: | 12 Feb 2020 07:12 |
| URI: | http://umpir.ump.edu.my/id/eprint/27643 |
| Download Statistic: | View Download Statistics |
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