Nurul Atikah, Mohd Rodzri and Salwani, Md Zain and Rafidah, Mohamed Ali Hanapiah and Rozaimi, Abu Samah and R. M., Illias (2020) D-Xylonic acid from recombinant e. coli BL21 (DE3): Comparison between shake flask and benchtop bioreactor fermentation. In: Journal of Physics: Conference Series, The 2nd Joint International Conference on Emerging Computing Technology and Sports (JICETS 2019) , 25-27 November 2019 , Bandung, Indonesia. pp. 1-8., 1529 (052073). ISSN 1742-6588 (print); 1742-6596 (online)
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Abstract
Production ᴅ-xylonic acid from recombinant E. coli BL21 (DE3) at a small scale has been successfully done several times. In an attempt to scale up the production process to a bigger scale, fermentation in bioreactor was conducted. The comparison on growth of recombinant E. coli BL21 (DE3) and production of ᴅ-xylonic acid in both shake flask and bioreactor was investigated. Higher specific growth rate of recombinant E. coli BL21 (DE3) and concentration of ᴅ-xylonic acid however, was obtained by fermentation in shake flask which is 0.273 h-1 and 9.06 gL-1 ᴅ-xylonic acid respectively. The result suggests several mistakes done during fermentation in bioreactor that causes low concentration of ᴅ-xylonic acid in bioreactor fermentation. Therefore, this calls up for improvement and better scale-up strategies in future work.
| Item Type: | Conference or Workshop Item (Lecture) |
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| Uncontrolled Keywords: | D-xylonic acid; Recombinant E. coli BL21 (DE3); Scale-up |
| Subjects: | T Technology > TP Chemical technology |
| Faculty/Division: | Institute of Postgraduate Studies Faculty of Chemical and Process Engineering Technology |
| Depositing User: | Pn. Hazlinda Abd Rahman |
| Date Deposited: | 09 Feb 2021 08:48 |
| Last Modified: | 09 Feb 2021 08:48 |
| URI: | http://umpir.ump.edu.my/id/eprint/29359 |
| Download Statistic: | View Download Statistics |
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