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Influence of Carbon Source and Culture Condition on Arachidonic Acid Production by Candida Krusei in Submerged Culture

Noraziah, Abu Yazid and Nurul Izyani, A.M. (2012) Influence of Carbon Source and Culture Condition on Arachidonic Acid Production by Candida Krusei in Submerged Culture. In: ISAET Proceedings: 2nd International Conference on Chemical, Biological and Environment Sciences 2012, 30 June - 1 July 2012 , Bali, Indonesia. pp. 1-4..

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Abstract

The growing interest in the application of arachidonic acid (AA) in pharmaceutical and dietary requirements has obtained much attention on production of AA by microorganism. The influence of carbon sources and culture condition on arachidonic acid production was investigated in cultures of Candida krusei using shake flasks. The optimum culture condition for high yield of AA was determined by varying the pH (in range of 5 to 9) and agitation speed (in range of 50 to 250 rpm). The carbon sources that were used are sucrose, starch and the mixture of sucrose and starch. The highest dry biomass concentration (0.1473 g/ml) was obtained under culture condition at pH 7 and 250 rpm agitation rate by using sucrose as a carbon source. While, the highest yield of AA (5.0916 g/ml) was produced at pH 7 and 150 rpm agitation rate by using sucrose as carbon source.

Item Type: Conference or Workshop Item (Paper)
Uncontrolled Keywords: Arachidonic acid; Candida krusei; Carbon source; lipids
Subjects: T Technology > TP Chemical technology
Faculty/Division: Faculty of Chemical & Natural Resources Engineering
Depositing User: Pn. Hazlinda Abd Rahman
Date Deposited: 21 Nov 2012 02:24
Last Modified: 15 May 2018 07:58
URI: http://umpir.ump.edu.my/id/eprint/3014
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