F. H., Zainuddin and Chua, Gek Kee and Chew, Few Ne and Rozaimi, Abu Samah and Siti Hatijah, Mortan and Noraziah, Abu Yazid (2020) Effect of type of nitrogen sources and concentration on protease production: fish waste hydrolysate as a potential low cost source. In: IOP Conference Series: Materials Science and Engineering; 5th International Conference of Chemical Engineering and Industrial Biotechnology, ICCEIB 2020 , 9 - 11 August 2020 , Kuala Lumpur, Malaysia. pp. 1-10., 991 (1). ISSN 1757-8981 (Print), 1757-899X (Online)
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Abstract
Fish waste hydrolysate is obtained from hydrolysis process of a fishery industry waste. It can be a good substrate source for the production of protease via a fermentation process. Protease covers above than 50% of the global industrial enzymes market and is regarded as an enzyme which is widely used in many applications. This research aims to determine the effect of the type of nitrogen sources and nitrogen source concentration on protease production from the fish waste hydrolysate. The experiments were done using one factor at a time (OFAT) of type of nitrogen sources and nitrogen source concentration in submerged fermentation (SmF) of 10% (v/v) Bacillus cereus (ATCC 10876). In the first stage, the variation of type of 1% (w/v) nitrogen sources such as fish waste hydrolysate, beef extracts, yeast extracts, tryptone, peptone and skim milk powder that mixed with the basal media were compared to evaluate the highest protease production. Then, the concentration of fish waste hydrolysate was varied from 1% to 3% (w/v) to analyse the production trend of protease. The protease activity and total protein content were then analysed by protease assay using tyrosine as a standard and Bradford method, respectively. Upon the observation, 1% (w/v) nitrogen sources on protease production revealed that the fish waste hydrolysates and beef extracts were capable to produce a high amount of protease (23.659 ± 0.38 U/mL and 28.7631 ± 0.59 U/mL, respectively). Among the concentration tested, 3% (w/v) of fish waste hydrolysate was found to be the highest protease production (190.85 ± 0.38 U/mL). Therefore, the fish waste hydrolysate has a potential as a low-cost source for protease production.
| Item Type: | Conference or Workshop Item (Lecture) |
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| Additional Information: | Indexed by Scopus |
| Uncontrolled Keywords: | Fish waste hydrolysate; Protease, submerged fermentation (SmF); Nitrogen sources; Nitrogen source concentrations |
| Subjects: | T Technology > TP Chemical technology |
| Faculty/Division: | Institute of Postgraduate Studies College of Engineering |
| Depositing User: | Mrs Norsaini Abdul Samat |
| Date Deposited: | 23 Jul 2021 08:19 |
| Last Modified: | 23 Jul 2021 08:19 |
| URI: | http://umpir.ump.edu.my/id/eprint/30932 |
| Download Statistic: | View Download Statistics |
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