S., Suhaimi and Rohaida, Che Man and Natassha, Jamil and Zatul Iffah, Mohd Arshad and Shalyda, Md Shaarani and Siti Kholijah, Abdul Mudalip and Siti Zubaidah, Sulaiman (2020) Optimization of reaction conditions for the production of cyclodextrin (CD) using cyclodextrin glucanotransferase (CGTase) immobilized on hollow fiber membrane. In: IOP Conference Series: Materials Science and Engineering, Energy Security and Chemical Engineering Congress , 17-19 July 2019 , Kuala Lumpur, Malaysia. pp. 1-8., 736 (042005). ISSN 1757-8981 (Print), 1757-899X (Online)
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Abstract
Cyclodextrin glucanotransferase (CGTase) is an enzyme that convert starch into cyclodextrin (CD) by transglycosylation reaction. The CD has been used in various industries due to the unique characteristics. However, the production of CD is usually restricted due to the instability of the enzyme which easily denatured during the reaction process. Thus, enzyme immobilization technique was applied and optimization of reaction conditions was conducted to enhance the amount of CD production. In this study, CGTase was immobilized on polyvinylidiene difluoride (PVDF) hollow fiber membrane by adsorption. The optimization of the reaction conditions using response surface methodology (RSM) on the production of CD was studied. Under optimized conditions (2.8% w/v of soluble potato starch concentration, 45.2°C of reaction temperature and pH 5.6), the production of CD was 5.65 mg/mL, about 2-fold compared to the value before optimization process. Therefore, the immobilized CGTase on hollow fiber membrane proved to be valuable for the enhancement of CD production.
| Item Type: | Conference or Workshop Item (Lecture) |
|---|---|
| Additional Information: | Indexed by Scopus |
| Uncontrolled Keywords: | Cyclodextrin glucanotransferase; Hollow fiber membrane; Enzyme immobilization |
| Subjects: | T Technology > TP Chemical technology |
| Faculty/Division: | Faculty of Industrial Sciences And Technology Institute of Postgraduate Studies Faculty of Chemical and Process Engineering Technology |
| Depositing User: | Mrs Norsaini Abdul Samat |
| Date Deposited: | 28 Apr 2021 04:10 |
| Last Modified: | 28 Apr 2021 04:10 |
| URI: | http://umpir.ump.edu.my/id/eprint/31169 |
| Download Statistic: | View Download Statistics |
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