Acceleration of Lipid Accumulation in Oleaginous Diatom Navicula sp. Under Nitrogen Limitation

Karthick Murugan, Palanisamy and Karthiani, Kanagesan and Mohd Hasbi, Ab. Rahim and Natanamurugaraj, Govindan and Gaanty Pragas, Maniam (2021) Acceleration of Lipid Accumulation in Oleaginous Diatom Navicula sp. Under Nitrogen Limitation. In: E-Proceeding of the 1st Postgraduate Seminar on Agriculture and Forestry: Enhancing Knowledge through Research (PSAF 2021) , Virtual Mode , 6 October 2021. . ISBN 978-967-26369

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Abstract

Diatom biomass gain attention globally as a source of lipid due to their high growth rate and biomass composition which can compete with fossil fuel for biodiesel production. Accumulations of natural lipid can be enhanced by various stress factors in diatoms. In this study, the effect of different nitrogen concentration on biomass and lipid production was investigated by cultivation of oleaginous diatom Navicula sp. for biodiesel production. The cultivation performed in phases where initially the culture was cultured in standard media for seven day and followed in different concentration of nitrogen such as limitation (0.35 mM), standard as media (1.76 mM), Repletion (3.5 mM) and free of nitrogen (0 mM) as second phase for eight days. The cells were grown under light intensity of 150 μmol photon m-2s-1(12:12 h day: light period) temperature (21±1 °C). Diatom Navicula sp. Showed highest growth and biomass production (734±15 mg/L) in nutrient f/2 standard media and extracted 19% of lipid. However, 24% of lipid yield was extracted which is significantly high from the biomass (528±10 mg/L) cultured in limited nitrogen media. Nitrogen limitation enhanced the fatty acid as saturated fatty acid C16:0, unsaturated fatty acid C16:1 and polyunsaturated fatty acid C18:3. Navicula sp., have potential to accumulate lipid in nitrogen limitation condition biodiesel production.

Item Type: Conference or Workshop Item (Lecture)
Uncontrolled Keywords: Biodiesel; biomass; diatom, Navicula sp.; nitrogen limitation
Subjects: Q Science > QD Chemistry
Faculty/Division: Faculty of Industrial Sciences And Technology
Institute of Postgraduate Studies
Depositing User: Noorul Farina Arifin
Date Deposited: 08 Dec 2021 08:29
Last Modified: 08 Dec 2021 08:30
URI: http://umpir.ump.edu.my/id/eprint/32792
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