Optimization of culture conditions of immobilized cells for enzyme excretion and cell lysis

Rohaida, Che Man and Rosli, Md Illias and Aizi Nor Mazila, Ramli and Siti Kholijah, Abdul Mudalip (2022) Optimization of culture conditions of immobilized cells for enzyme excretion and cell lysis. Chemical Engineering and Technology, 45 (8). 1461 -1466. ISSN 1521-4125. (Published)

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Abstract

Recombinant enzyme excretion is essential due to stable and active protein production. However, enzyme excretion, especially in the Escherichia coli system, may increase the occurrence of cell lysis. Thus, cell immobilization was used, and optimization of culture conditions was conducted to increase enzyme excretion and limit cell lysis. The culture conditions were optimized using a response surface methodology. CGTase excretion and cell lysis were analyzed by CGTase and β-galactosidase assays, respectively. High CGTase excretion with low cell lysis was achieved after the optimization process. The results suggest that optimization of the cell immobilization system increased CGTase excretion with low cell lysis and made it more valuable for industrial applications.

Item Type:	Article
Additional Information:	Indexed by Scopus
Uncontrolled Keywords:	Cell lysis; CGTase excretion; Free cell; Immobilized cell; Optimization
Subjects:	T Technology > TP Chemical technology
Faculty/Division:	Faculty of Industrial Sciences And Technology College of Engineering
Depositing User:	Pn. Hazlinda Abd Rahman
Date Deposited:	16 Jun 2022 03:04
Last Modified:	19 Apr 2023 01:40
URI:	http://umpir.ump.edu.my/id/eprint/33066
Download Statistic:	View Download Statistics

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