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Effect of substrate concentration and ph on succinic acid production

Nik Nor Aziati, Abd Aziz (2010) Effect of substrate concentration and ph on succinic acid production. Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang.


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The purpose of this research is to study the effect of substrate concentration and pH on succinic acid production. Succinic acid has been produced from the fermentation process by using Escherichia coli b-strain. The experiments were conducted in Modified Schott Bottle in incubator shaker to maintain anaerobic condition, temperature and agitation speed. Fermentation were carried out at the optimum temperature of E.coli growth which is 37 OC for 72 hours. The results of HPLC analysis showed that increase in fermentation time will increase succinic acid production. The maximum production of succinic acid is at pH 6.0. Initial substrate concentration (5 to 15 g/L) will increase Succinic acid production, but after 15 g/L substrate concentration, the succinic acid production will decrease. From this study, it was observed that the substrate concentration and pH are important factor that affects both growth and growth-associated productions of molecules to increased succinic acid production.

Item Type: Undergraduates Project Papers
Additional Information: Project paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang - 2010
Uncontrolled Keywords: Fermentation Succinic acid
Subjects: T Technology > TP Chemical technology
Faculty/Division: Faculty of Chemical & Natural Resources Engineering
Depositing User: Shamsor Masra Othman
Date Deposited: 16 May 2013 04:14
Last Modified: 03 Mar 2015 08:00
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