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Production of poly-hydroxy butyrate (PHB) by Bacilius Cereus using banana stem as a substrate

Nurul Hanim, Mat Noor (2010) Production of poly-hydroxy butyrate (PHB) by Bacilius Cereus using banana stem as a substrate. Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang.

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Abstract

The research was focused on delignification process and production of (Poly-β-hydroxybutyrate) PHB by Bacillus cereus using banana stem wastes as substrate. This study is divided into three parts which are determination growth curve of Bacillus cereus, lignin analysis using Klason method and determination of PHB. Delignification process is functioning to degrade lignin from banana stem to get cellulose that can be used as carbon sources in production of PHB. PHB is a carbon storage polymer that is widely distributed among prokaryotes including Bacilius cereus. The delignification methods are divided into three parts which are preparation of raw material and microorganisms, fermentation process and lignin analysis. Fermentation was carried out aerobically at optimum temperature 37 ˚C and 250 rpm of agitation speed for 2 days. In this process, Klason method was used to identify the percentage of lignin degraded. Based on the analysis, it shows that Bacillus cereus is able to degrade lignin from banana stem wastes. The average lignins degrade by Bacillus cereus was 40.175%. Meanwhile, for production of PHB methods were divided into four parts which are fermentation, pre-treatment, extraction and analysis. In extraction process, ultrasonic treatment, H2SO4, HCl and chloroform was used to break the granule and cell wall. In this study, the best PHB production and percentage yield of this strain was determined. The PHB production was 27.897 mg/ml and the percentage yield was 55.48% at 48 hours. After 48 hours there was a decrease in PHB yield because of the bacteria used PHB as a source of carbon and nitrogen, causing an unsuitable condition due to inadequate nitrogen and carbon sources in the medium.

Item Type: Undergraduates Project Papers
Uncontrolled Keywords: Fermentation Poly-beta-hydroxyalkanoates
Subjects: T Technology > TP Chemical technology
Faculty/Division: Faculty of Chemical & Natural Resources Engineering
Depositing User: Shamsor Masra Othman
Date Deposited: 16 May 2013 04:16
Last Modified: 03 Mar 2015 08:01
URI: http://umpir.ump.edu.my/id/eprint/3421
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