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ITS1 amplicon sequencing of feline gut mycobiome of Malaysian local breeds using Nanopore Flongle

Tay, Darren Dean and Siew, Shing Wei and Shamrulazhar, Shamzir Kamal and Mohd Najib, Razali and Hajar Fauzan, Ahmad (2022) ITS1 amplicon sequencing of feline gut mycobiome of Malaysian local breeds using Nanopore Flongle. Archives of Microbiology, 204 (6). pp. 1-11. ISSN 0302-8933

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Abstract

The gut mycobiome exhibits major influence on the gastrointestinal health and disease but received less attention due to low abundance. This study characterizes the fungal community and compares the microbial diversity between indoor and outdoor cats. Genomic DNA was extracted and sequenced by targeting the Internal Transcribed Spacer 1 (ITS1) region using Flongle flow cell on MinION™ sequencing platform. Results show the phylum Ascomycota and genus Peniophorella were numerous in indoor cats, whereas the Basidiomycota and Pichia were abundant in outdoor cats. Peniophorella formed the core mycobiome in both feline populations. Furthermore, alpha (p value = 0.0207) and beta diversities (p value = 0.009) results showed significant differences between the two groups. Overall, indoor cats have greater amounts of Peniophorella, whereas outdoor cats have higher Trichosporon and unclassified Sordariaceae. The study also suggests that keeping a cat indoors or left as a stray will affect their respective gut mycobiome.

Item Type: Article
Additional Information: Indexed by Scopus
Uncontrolled Keywords: Feline gut; Flongle; Fungi; Malaysia; Mycobiome
Subjects: Q Science > QP Physiology
T Technology > TP Chemical technology
Faculty/Division: Faculty of Industrial Sciences And Technology
Institute of Postgraduate Studies
Faculty of Chemical and Process Engineering Technology
Depositing User: Mrs Norsaini Abdul Samat
Date Deposited: 01 Jul 2022 03:23
Last Modified: 01 Jul 2022 03:23
URI: http://umpir.ump.edu.my/id/eprint/34570
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