Nur Fathin Shamirah, Daud (2021) Optimization and kinetic study of pigments production on solid state fermentation using oil palm frond by monascus purpureus ftc 5357. Masters thesis, Universiti Malaysia Pahang (Contributors, UNSPECIFIED: UNSPECIFIED).
|
Pdf
Optimization and kinetic study of pigments production on solid state fermentation using oil palm frond.ir.pdf - Accepted Version Download (441kB) | Preview |
Abstract
Monascus sp. produce useful Monascus pigments that widely used in food industry. Extensive study available on Monascus sp. in solid-state fermentation (SSF) using shake flask, however, cultivation of Monascus sp. in stirred drum bioreactor is insufficiently reported. Thus, this study aims to optimize pigments production (i.e. yellow, orange and red) from Monascus purpureus FTC 5357 using oil palm frond (OPF) in 5 L stirred-drum bioreactor. Prior to the SSF process, hydrothermal treatment (121 °C, 15 min) was done. A three-level Box–Behnken design (BBD) was used for the optimization, with threeparameters such as initial moisture content (IMC), aeration rate (AR) and peptone concentration (PC). The dried fermented OPF were extracted and analysed for pigments production (i.e. yellow, orange and red), biomass and glucose concentration. Next, the kinetic profile of the optimize condition and kinetic parameters were also been evaluated. Based on the ANOVA analysis results, IMC, AR and PC contributed significantly to the pigments production. The optimal fermentation conditions resulted IMC at 70 % w/w, AR at 1.30 vvm and PC at 4.40 % w/w. Under these conditions, the pigments production were obtained at 22.03, 20.11 and 18.59 Au/g.d for yellow, orange and red, respectively. Validation experiment was done to confirm the adequacy of the models. The fermentation profile at optimized condition indicated that Monascus purpureus FTC 5357 reached maximum pigments and biomass at day 9. Results obtained from this study showed the importance of IMC, AR and PC for pigments production and biomass. Meanwhile, the production kinetic parameters such as maximum specific growth rate, the growth yield, pigment yield, cell productivity, pigments productivity for yellow, orange and red were 0.707 day-1, 0.15 mg/mg, 0.193 Au/mg cell and 12.514 mg/(g.day), 2.447 Au/(g.d.day), 2.234 Au/(g.d.day) and 2.065 Au/(g.d.day), respectively. The pigments produced through SSF process using OPF as a substrate by Monascus purpureus FTC 5357 has a great potential to be utilized as a source of pigment for food in future.
| Item Type: | Thesis (Masters) |
|---|---|
| Additional Information: | Thesis (Master of Science) -- Universiti Malaysia Pahang – 2021, SV: ASSOCIATE PROFESSOR TS. DR. FARHAN BT MOHD SAID, NO. CD: 13042 |
| Uncontrolled Keywords: | kinetic study, pigments production, solid state fermentation, oil palm frond, monascus purpureus ftc 5357 |
| Subjects: | Q Science > QD Chemistry T Technology > TA Engineering (General). Civil engineering (General) |
| Faculty/Division: | Institute of Postgraduate Studies Faculty of Chemical and Process Engineering Technology |
| Depositing User: | Mr. Nik Ahmad Nasyrun Nik Abd Malik |
| Date Deposited: | 14 Oct 2022 01:55 |
| Last Modified: | 14 Oct 2022 01:55 |
| URI: | http://umpir.ump.edu.my/id/eprint/35302 |
| Download Statistic: | View Download Statistics |
Actions (login required)
 |
View Item |