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Factors Affecting Poly(3-hydroxybutyrate) Product Ion from Oil Palm Frond Juice by Cupriavidus Necator (CCUG52238T)

Mior Ahmad Khushairi, Mohd Zahari and Jailani, Salihon (2012) Factors Affecting Poly(3-hydroxybutyrate) Product Ion from Oil Palm Frond Juice by Cupriavidus Necator (CCUG52238T). Journal of Biomedicine and Biotechnology, 2012.

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Abstract

Factors influencing poly(3-hydroxybutyrate) P(3HB) production by  Cupriavidus necator CCUG52238T utilizing oil palm frond (OPF) juice were clarified in this study. Effects of initial medium pH, agitation speed, and ammonium sulfate (NH4)2SO4 concentration on the production of P(3HB) were investigated in shake flasks experiments using OPF juice as the sole carbon source. The highest P(3HB) content was recorded at pH 7.0, agitation speed of 220 rpm, and (NH4)2SO4 concentration at 0.5 g/L. By culturing the wild-type strain of C. necator under the aforementioned conditions, the cell dry weight (CDW) and P(3HB) content obtained were 9.31 ± 0.13 g/L and 45 ± 1.5 wt.%, respectively. This accounted for 40% increment of P(3HB) content compared to the nonoptimized condition. In the meanwhile, the effect of dissolved oxygen tension (DOT) on P(3HB) production was investigated in a 2-L bioreactor. Highest CDW (11.37 g/L) and P(3HB) content (44 wt.%) were achieved when DOT level was set at 30%. P(3HB) produced from OPF juice had a tensile strength of 40 MPa and elongation at break of 8% demonstrated that P(3HB) produced from renewable and cheap carbon source is comparable to those produced from commercial substrate.

Item Type: Article
Additional Information: Article ID 125865, 8 pages doi:10.1155/2012/125865 Please click to official URL
Uncontrolled Keywords: Oil palm frond (OPF), Poly(3-hydroxybutyrate) production;
Subjects: T Technology > TP Chemical technology
Faculty/Division: Faculty of Chemical & Natural Resources Engineering
Depositing User: Noorul Farina Arifin
Date Deposited: 06 Jun 2013 03:38
Last Modified: 19 Feb 2018 06:43
URI: http://umpir.ump.edu.my/id/eprint/3784
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