Nur Fathin Shamirah, Daud and Farhan, Mohd Said and M. A., Mokhtar (2023) Optimization of solvent systems for color fractionation of monascus pigment. Journal of Chemical Engineering and Industrial Biotechnology (JCEIB), 9 (2). pp. 41-47. ISSN 0126-8139. (Published)
|
Pdf
Optimization of Solvent Systems for Color Fractionation.pdf Available under License Creative Commons Attribution Non-commercial. Download (462kB) | Preview |
Abstract
Monascus fungi are unique among the various microorganisms that produce edible pigments, which consist of red, orange, and yellow. These pigments are typically present in a mixture. Hence, pigment separation is needed. This study aims to optimize solvent systems that can effectively fractionate the pigments. The optimization was carried out using column chromatography and the data was analyzed using Central Composite Design (CCD). The interaction of the solvent systems ethyl acetate : formic acid : acetic acid : water was optimized. The optimal solvent system obtained was 98 : 14 : 12 : 28 (vol/vol.) for ethyl acetate : formic acid : acetic acid : water, respectively. The red and yellow pigment obtained were 2.42 mg and 0.93 mg, respectively. Based on the finding, 72.2% of the total Monascus pigments was red while remaining was yellow.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Central composite design; Fractionation; Monascus pigments; Thin layer; chromatography |
| Subjects: | T Technology > TP Chemical technology |
| Faculty/Division: | Institute of Postgraduate Studies Faculty of Chemical and Process Engineering Technology |
| Depositing User: | Noorul Farina Arifin |
| Date Deposited: | 19 Feb 2024 07:45 |
| Last Modified: | 19 Feb 2024 07:45 |
| URI: | http://umpir.ump.edu.my/id/eprint/38658 |
| Download Statistic: | View Download Statistics |
Actions (login required)
 |
View Item |