UMP Institutional Repository

Preparation of cation-exchange membrane chromatography through modification of polyamide membrane by grafting of acrylic acid monomer

Nur Aishah Amalin, Ramjis (2012) Preparation of cation-exchange membrane chromatography through modification of polyamide membrane by grafting of acrylic acid monomer. Faculty of Chemical & Natural Resource Engineering, Universiti Malaysia Pahang.

[img]
Preview
PDF
CD6417.pdf

Download (2MB)

Abstract

Packed bed chromatography is widely used for protein separation and purification.However some limitation of packed bed chromatography has been identified such as high-pressure drop,internal diffusion limitation and compaction for the soft beads.This encourages people to use membrane chromatography as alternative to the packed bed chromatography.In this study,membrane chromatography was developed from polyamide-based membrane by grafting acrylic acid (AA) monomer to produce cation-exchanger membrane chromatography.During grafting process,the concentration of AA monomer from 0.1 M to 1.0 M and grafting time from 15 min to 120 min was studied.The optimum monomer concentration was achieved at 0.5 M with a binding capacity of 443.39 mg BSA/g membrane.The optimum reaction time is 45 minutes which gives a binding capacity of 533.40 BSA mg/g membranes.The BSA adsorption capacity can be related to the amount of carboxyl group present in the modified membrane surface after grafting process.The successful of membrane chromatography preparation depend on the careful optimization the chemical reaction parameters involve.

Item Type: Undergraduates Project Papers
Uncontrolled Keywords: Chromatography
Subjects: T Technology > TP Chemical technology
Faculty/Division: Faculty of Chemical & Natural Resources Engineering
Depositing User: Shamsor Masra Othman
Date Deposited: 08 Jul 2014 03:39
Last Modified: 03 Mar 2015 09:22
URI: http://umpir.ump.edu.my/id/eprint/4995
Download Statistic: View Download Statistics

Actions (login required)

View Item View Item