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Enzymatic Production of Bioxylitol from Sawdust Hydrolysate: Screening of Process Parameters

M. Rafiqul, Islam and Mimi Sakinah, A. M. and Zularisam, A. W. (2015) Enzymatic Production of Bioxylitol from Sawdust Hydrolysate: Screening of Process Parameters. Applied Biochemistry and Biotechnology, 176 (4). pp. 1071-1083. ISSN 1559-0291

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Abstract

Xylose-rich sawdust hydrolysate can be an economic substrate for the enzymatic production of xylitol, a specialty product. It is important to identify the process factors influencing xylitol production. This research aimed to screen the parameters significantly affecting bioxylitol synthesis from wood sawdust by xylose reductase (XR). Enzymatic bioxylitol production was conducted to estimate the effect of different variables reaction time (2–18 h), temperature (20–70 °C), pH (4.0–9.0), NADPH (1.17–5.32 g/L), and enzyme concentration (2–6 %) on the yield of xylitol. Fractional factorial design was followed to identify the key process factors. The screening design identified that time, temperature, and pH are the most significant factors influencing bioxylitol production among the variables with the values of 12 h, 35 °C, and 7.0, respectively. These conditions led to a xylitol yield of 71 % (w/w). This is the first report on the statistical screening of process variables influencing enzyme-based bioxylitol production from lignocellulosic biomass.

Item Type: Article
Uncontrolled Keywords: Bioconversion; Hemicellulosic hydrolysate; Screening design Bioxylitol; Xylose; Xylose reductase
Subjects: T Technology > TP Chemical technology
Faculty/Division: Faculty of Chemical & Natural Resources Engineering
Depositing User: Mrs. Neng Sury Sulaiman
Date Deposited: 04 Nov 2015 06:43
Last Modified: 29 Nov 2018 07:25
URI: http://umpir.ump.edu.my/id/eprint/6191
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