Application of Adsorption Chromatography in the Papain Processing

He, Yuhai and Syed Mohd Saufi, Tuan Chik and Chong, Fui Chin (2014) Application of Adsorption Chromatography in the Papain Processing. GSTF Journal of Chemical Sciences (JChem), 1 (2). pp. 9-15. ISSN 2339-5079 (Online). (Published)


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A direct recovery of papain enzyme from unclarified Carica papaya juice was developed successfully using a reversed phase expanded bed adsorption chromatography (RP-EBAC). The dynamic binding capacity for the RP-EBAC at 10% breakthrough of 810.52 μg papain/g adsorbent was achieved at a linear flow velocity of 936 cm/h, bed expansion of two and feedstock viscosity of 2.68 mPa· s. Two-step elution was employed to enhance the purity of papain in RP-EBAC which gives high papain purity of 74.98% and high purification factor of 7.04. This work shows a great potential of using RP-EBAC with elution optimization step to purify papain from unclarified Carica papaya juice.

Item Type: Article
Uncontrolled Keywords: Papain, Chromatography, RP-EBAC, Purification
Subjects: T Technology > TP Chemical technology
Faculty/Division: Faculty of Chemical & Natural Resources Engineering
Depositing User: Mrs. Neng Sury Sulaiman
Date Deposited: 18 Feb 2016 06:00
Last Modified: 26 Mar 2018 07:33
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