Targeting N-acyl-homoserine-lactones to Mitigate Membrane Biofouling based on Quorum Sensing using a Biofouling Reducer

Muhammad Faisal, Siddiqui and Mimi Sakinah, A. M. and Singh, Lakhveer and Zularisam, A. W. (2012) Targeting N-acyl-homoserine-lactones to Mitigate Membrane Biofouling based on Quorum Sensing using a Biofouling Reducer. Journal of Biotechnology, 161. pp. 190-197. ISSN 0168-1656. (Published)

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Abstract

Exploring novel biological anti-quorum sensing (QS) agents to control membrane biofouling is of great worth in order to allow sustainable performance of membrane bioreactors (MBRs) for wastewater treatment. In recent studies, QS inhibitors have provided evidence of alternative route to control membrane biofouling. This study investigated the role of Piper betle extract (PBE) as an anti-QS agent to mitigate membrane biofouling. Results demonstrated the occurrence of the N-acyl-homoserine-lactone (AHL) autoinducers (AIs), correlate QS activity and membrane biofouling mitigation. The AIs production in bioreactor was confirmed using an indicator strain Agrobacterium tumefaciens (NTL4) harboring plasmid pZLR4. Moreover, three different AHLs were found in biocake using thin layer chromatographic analysis. An increase in extracellular polymeric substances (EPS) and transmembrane pressure (TMP) was observed with AHL activity of the biocake during continuous MBR operation, which shows that membrane biofouling was in close relationship with QS activity. PBE was verified to mitigate membrane biofouling via inhibiting AIs production. SEM analysis further confirmed the effect of PBE on EPS and biofilm formation. These results exhibited that PBE could be a novel agent to target AIs for mitigation of membrane biofouling. Further work can be carried out to purify the active compound of Piper betle extract to target the QS to mitigate membrane biofouling.

Item Type:	Article
Additional Information:	Zularisam Ab Wahid (A. W. Zularisam)
Uncontrolled Keywords:	Quorum sensing, Membrane biofouling, Piper betle extract, N-Acyl-homoserine-lactone, Autoinducers
Subjects:	T Technology > TA Engineering (General). Civil engineering (General)
Faculty/Division:	Faculty of Chemical & Natural Resources Engineering Faculty of Civil Engineering & Earth Resources Faculty of Industrial Sciences And Technology
Depositing User:	Ms Suriati Mohd Adam
Date Deposited:	04 Apr 2016 04:19
Last Modified:	17 Jan 2018 01:05
URI:	http://umpir.ump.edu.my/id/eprint/6817
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