Purification and Partial Characterization of a Low Molecular Weight L-Asparaginase Produced from Corn Cob Waste

Makky, Essam A. and Yean , Chun Loh and Karim, Md. Rezaul (2014) Purification and Partial Characterization of a Low Molecular Weight L-Asparaginase Produced from Corn Cob Waste. Biocatalysis and Agricultural Biotechnology, 3 (4). pp. 265-270. ISSN 1878-8181. (Published)

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DOI/Official URL: http://dx.doi.org/10.1016/j.bcab.2014.07.004

Abstract

l-asparaginase (E.C.3.5.1.1) is an important enzyme often used to treat acute lymphoblastic leukemia. This paper describes the production, purification and partial characterization of l-asparaginase by Streptobacillus sp. KK2S4. The highest l-asparaginase production was achieved with 0.2% (w/v) of lactose, 0.1% (w/v) of sodium nitrate, 6% (w/v) of pre-treated corn cob powder and 4% (v/v) of KK2S4 bacterial suspension in 50 ml of MM9 media at pH 5 and 40 °C. The enzyme was purified by ammonium sulfate precipitation followed by DEAE-cellulose and Sephadex G-50 column chromatography. The specific activity of the pure enzyme was recorded to be 21.77 U/mg with 39.58-fold purification and 39% of yield. SDS-PAGE demonstrated a single band with molecular weight of 11.2 kDa. The optimum activity of purified enzyme was recorded at pH 8.5 and 35 °C. This is the first report of a very low molecular weight microbial l-asparaginase produced from corn cob as the main carbon source.

Item Type:	Article
Uncontrolled Keywords:	Corn cob; Microbial l-asparaginase; Purity; Molecular weight
Subjects:	Q Science > Q Science (General)
Faculty/Division:	Faculty of Industrial Sciences And Technology
Depositing User:	Mrs. Neng Sury Sulaiman
Date Deposited:	18 Dec 2014 02:35
Last Modified:	09 Jan 2018 07:52
URI:	http://umpir.ump.edu.my/id/eprint/7034
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