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Fermentative Production of Butanol From Oil Palm Trunk (OPT) Sap Using Clostridium Acetobutylicum and Clostridium Beijerinckii

Farhanah, Amanulla Khan (2013) Fermentative Production of Butanol From Oil Palm Trunk (OPT) Sap Using Clostridium Acetobutylicum and Clostridium Beijerinckii. Faculty of Chemical and Natural Resources Engineering, Universiti Malaysia Pahang.


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Malaysia is the one of world’s largest producer of palm oil. Waste from palm oil production factories has been increasing rapidly and one of the serious problems in the palm oil fruit processing is managing the waste generated by the processes. Recent studies showed that butanol can be used as excellent renewable liquid transportation fuel. In this study, oil palm trunk sap is used as the substrate. The objective of this study is to compare the production of biobutanol from biomass waste (oil palm trunk sap) via fermentation process using different microorganisms. The fermentation type done was submerged fermentation. Fermentation was conducted for five days at 37 ⁰C. Samples were taken for every 6 hours for 3 days, then for every 12 hours. Samples taken were analyzed using gas chromatography-flame ionization detector (GC-FID) and high performance liquid chromatography (HPLC) was used to analyze sugar composition in OPT sap. UV Spectrophotometer is used to analyze biomass. Oil palm trunk (OPT) sap contains sucrose as its dominant sugar followed by glucose, fructose and galactose. Based on the analysis, acetone, butanol and ethanol were produced. Comparisons between the two strains Clostridium acetobutylicum and Clostridium beijerinckii were made, and C. beijerinckii showed higher production of acetone, butanol and ethanol. The highest yield for butanol production by C. acetobutylicum and C. beijerinckii were 13.910 g/L and 15.301 g/L and highest production of ethanol were 11.17 g/L and 12.09 g/L. 4.13 g/L and 4.49 g/L acetone was produced by C. acetobutylicum and C. beijerinckii. Based on this study, it is concluded that OPT sap is preferable substrate to produce butanol by using C. beijerinckii.

Item Type: Undergraduates Project Papers
Additional Information: Project paper (Bachelor of Chemical Engineering (Biotechnology)) -- Universiti Malaysia Pahang – 2013
Uncontrolled Keywords: Fermentation Butanol Oil palm
Subjects: T Technology > TP Chemical technology
Depositing User: Ms Suriati Mohd Adam
Date Deposited: 14 Oct 2014 07:16
Last Modified: 03 Mar 2015 09:34
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