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Chemical Characterization and Biological Study of Azadirachta indica Extracts

Jessinta, Sandanasamy and Azhari, H. Nour and Saiful Nizam, Tajuddin and Nour, A. H. (2014) Chemical Characterization and Biological Study of Azadirachta indica Extracts. European Journal of Academic Essays, 1 (10). pp. 9-16. ISSN 2183-3818(Print); 2183-1904(Online)

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Abstract

The utilization of the plant parts (bark, leaves and roots) of Azadirachta indica from Malaysia for research purpose were limited and most of the previous studies focused only on the leaves. Thus, this study focused on the volatile compounds and the cytotoxic study of crude and fractions obtained from bark, leaves and roots of A. indica. The analysis of the volatiles of the crude and fractions was performed via Gas Chromatography-Mass Spectrometry (GC-MS). The volatiles present vary according to the plant parts and extraction solvents. The bark aqueous fraction yielded highest amount of volatiles (19 compounds) that corresponds towards various reported activities. The n-hexadecanoic acid was the most frequently occurring compound detected in all extracts, bark (40.14 and 20.1%), leaf (65.18 and 44.79%) and root (20.55 and 9.14%) for crudes and fractions, respectively. Meanwhile, the cytotoxic bioassay against brine shrimp (Artemia salina) resulted that; the fractions were more toxic compared to the crude extracts. Among all the extracts, leaf ethyl acetate fraction provides a lethal concentration value (1.35 ± 0.40 ppm) of the lowest, followed by bark ethyl acetate (1.38 ± 0.33) and leaf chloroform fraction (2.14 ± 0.35 ppm) and thus indicating the highest cytotoxic effect. The bioassay proved that fractions provide a better extraction technique for the extraction of toxic phytochemicals compared to the crude extracts

Item Type: Article
Uncontrolled Keywords: Azadirachta indica, Crude, Fractions, Volatile Compound, Cytotoxic Bioassay
Subjects: Q Science > Q Science (General)
T Technology > TP Chemical technology
Faculty/Division: Faculty of Chemical & Natural Resources Engineering
Faculty of Industrial Sciences And Technology
Depositing User: Mrs. Neng Sury Sulaiman
Date Deposited: 17 Dec 2014 07:01
Last Modified: 24 Jan 2018 06:46
URI: http://umpir.ump.edu.my/id/eprint/7487
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