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Optimisation of Bioethanol Yield from Oil Palm Trunk Sap

Rossyuhaida, Mohd Zakria and Chua, Gek Kee and Jolius, Gimbun and Nor Shahirah, Mohd Nasir and Sook, Fun Pang and Mohd Fazli, Farida Asras and Cheng, C. K. and Ahmad Ziad, Sulaiman (2015) Optimisation of Bioethanol Yield from Oil Palm Trunk Sap. In: Proceeding Book Mucet 2014 : Engineering & Technology. Universiti Teknikal Malaysia Melaka (UTeM), Melaka, pp. 340-346. ISBN 978-967-0257-53-2

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This paper presents the effect of nutrients addition and fermentation microorganism on bioethanol yield from oil palm trunk sap. Six microorganisms namely, Saccharomyces cerevisiae ATCC 9763, Saccharomyces cerevisiae ATCC 26602, baker’s yeast, Kluyveromyces marxianus ATCC 46537, Zymomonas mobilis ATCC 29501 and Escherichia coli ATCC 10536 were screened for ethanol production at fixed temperature, pH, agitation and inoculum size. The sugar and ethanol content were determined using a high performance liquid chromatography (HPLC). The results showed that K. marxianus produced the highest ethanol yield (60.9%) at a shorter fermentation time (16h) compared to the other strains. Six nutrients, namely, ammonium sulphate, di-ammonium hydrogen phosphate, magnesium sulphate, β-alanine, calcium chloride and potassium dihydrogen phosphate were screened using this strain and the highest ethanol yield (98.62%) was achieved in fermentation supplemented by magnesium sulphate and β-alanine. Subsequently, the optimisation study using a reseponse surfae methodology found the optimum value of magnesium sulphate was 7.93 g/L and 0.90 g/L for β-alanine. Under the optimum conditions, the predicted ethanol concentration was 34.58 g/L while the experimental value (35.50 g/L) was in agreement with the predicted value with 2.66% error.

Item Type: Book Section
Additional Information: 8th Malaysian Technical Universities Conference on Engineering & Technology (MUCET 2014), 10-11 November 2014, Mahkota Hotel Bandar Hilir Melaka.
Uncontrolled Keywords: Bioethanol; Oil Palm Trunk Sap; Nutrient Supplementation; Response Surface Methodology
Subjects: T Technology > TP Chemical technology
Faculty/Division: Faculty of Chemical & Natural Resources Engineering
Faculty of Industrial Sciences And Technology
Depositing User: Mrs. Neng Sury Sulaiman
Date Deposited: 10 Dec 2014 00:40
Last Modified: 10 Jan 2018 07:43
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