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Optimal C:N ratio for the production of red pigments by Monascus ruber

M. Said, Farhan and Brooks, John and Chisti, Yusuf (2014) Optimal C:N ratio for the production of red pigments by Monascus ruber. World Journal of Microbiology and Biotechnology, 30 (9). pp. 2471-2479. ISSN 0959-3993

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Abstract

The carbon-to-nitrogen (C:N) ratio in the biomass of microfungi tends to be quite different (e.g. 10–15) compared with the C:N ratio in the red pigments (e.g.[20) of the fungus Monascus ruber. Therefore, determining an optimal C:N ratio in the culture medium for maximizing the production of the pigments is important. A culture medium composition is established for maximizing the production of the red pigment by the fungus M. ruber ICMP 15220 in submerged culture. The highest volumetric productivity of the red pigment was 0.023 AU L-1 h-1 in a batch culture (30 C, initial pH of 6.5) with a defined medium of the following composition (g L-1): glucose (10), monosodium glutamate (MSG) (10), MgSO47H2O (0.5), KH2PO4 (5), K2HPO4 (5), ZnSO47H2O (0.01),FeSO47H2O (0.01), CaCl2 (0.1), MnSO4H2O (0.03). This medium formulation had a C:N mole ratio of 9:1. Under these conditions, the specific growth rate of the fungus was 0.043 h-1 and the peak biomass concentration was 6.7 g L-1 in a 7-day culture. The biomass specific productivity of the red pigment was 1.06 AU g-1 h-1. The best nitrogen source proved to be MSG although four other inorganic nitrogen sources were evaluated.

Item Type: Article
Uncontrolled Keywords: onascus ruber; Food colorants; Red pigments; Submerged culture
Subjects: T Technology > TP Chemical technology
Faculty/Division: Faculty of Chemical & Natural Resources Engineering
Depositing User: Dr. Farhan Mohd. Said
Date Deposited: 08 Jan 2015 04:09
Last Modified: 04 Apr 2018 05:48
URI: http://umpir.ump.edu.my/id/eprint/8089
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