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Effects of pH and temperature on glucose production from tapioca starch using enzymatic hydrolysis : a statistical approach

Siti Nor Shadila, Alias (2009) Effects of pH and temperature on glucose production from tapioca starch using enzymatic hydrolysis : a statistical approach. Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang.

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Abstract

The aim of this study was to maximize the glucose concentration produced from enzymatic hydrolysis of tapioca starch. The tapioca starch was enzymatically hydrolyzed using α-amylase from Bacillus lichenformis followed by amyloglucosidase action from Aspergillus niger. Effects of liquefaction temperature (X1), saccharification temperature (X2), liquefaction pH (X3) and saccharification pH (X4¬) were evaluated. 24 full factorial design with 1 replicate and 3 centered point was applied to determine the significant parameters affecting the production of glucose concentration. The range of the factors employed were 60 -90˚C (liquefaction temperature), 40-60˚C (saccharification temperature), 5-7 (liquefaction pH) and 4-6 (saccharification pH). The maximum glucose concentration obtained experimentally was 329.10 g/L. The ANOVA shows that the effects of liquefaction temperature and saccharification pH on glucose production were very significant. The saccharification temperature and liquefaction pH, on the other hand did not influence the glucose production. The optimum liquefaction temperature, saccharification temperature, liquefaction pH and saccharification pH suggested by the design of experiment were 90˚C, 60˚C, 7 and 6 respectively. From that optimum condition, the maximum glucose concentration of 331.8 g/L was estimated.

Item Type: Undergraduates Project Papers
Additional Information: For FULL TEXT, email umplibrary.ir@gmail.com enclosed with full name, institution, state, telephone number, position (student, lecturer, researcher) and the purpose of thesis is required.
Uncontrolled Keywords: Glucose
Subjects: T Technology > TP Chemical technology
Faculty/Division: Faculty of Chemical & Natural Resources Engineering
Depositing User: Rosfadilla Mohamad Zainun
Date Deposited: 22 Aug 2010 23:22
Last Modified: 03 Mar 2015 07:47
URI: http://umpir.ump.edu.my/id/eprint/863
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