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Evaluation of Sawdust Hemicellulosic Hydrolysate for Bioproduction of Xylitol by Enzyme Xylose Reductase

M. Rafiqul, Islam and Mimi Sakinah, A. M. and Zularisam, A. W. (2015) Evaluation of Sawdust Hemicellulosic Hydrolysate for Bioproduction of Xylitol by Enzyme Xylose Reductase. Food and Bioproducts Processing, 94. pp. 82-89. ISSN 0960-3085

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Abstract

Sawdust hemicellulosic hydrolysate can be used as a promising xylose source for enzymatic conversion to xylitol, a specialty product. The aim of this study was to design various factors by observing their effects on xylitol production from hemicellulosic hydrolysate by xylose reductase (XR) enzyme. The one-factor-at-a-time (OFAT) strategy was adopted to determine the effective levels of process factors influencing xylitol yield. Enzymatic xylitol production was conducted in batch mode using different levels of reaction time (2–18h), temperature (20–70 ◦C), pH (4.0–9.0), xylose concentration (9.4–37.6 g/L), NADPH concentration (1.17–5.32 g/L), enzyme concentration (2–6%), and agitation (50–150 rpm). Among the factors examined, time, temperature, pH, NADPH, and enzyme concentration significantly influenced the biosynthesis of xylitol with the suitable level of 10h, 30 ◦C, 7.0, 3.66 g/L, and 3% (v/v), respectively. Xylitol yield obtained was 56% (w/w) under these observed optimum conditions. This is the first report on the bioproduction of xylitol from lignocellulosic substrate by XR.

Item Type: Article
Uncontrolled Keywords: Biotransformation; Hemicellulosic hydrolysate; Parameter design; Xylitol; Xylose; Xylose reductase
Subjects: T Technology > TP Chemical technology
Faculty/Division: Faculty of Chemical & Natural Resources Engineering
Depositing User: Mrs. Neng Sury Sulaiman
Date Deposited: 23 Jul 2015 01:59
Last Modified: 29 Nov 2018 07:25
URI: http://umpir.ump.edu.my/id/eprint/8808
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