A. K. M., Kafi and N. S., Azmi and M. M., Yusoff and Crossley, Maxwell J. (2017) Superoxide Radical Biosensor Based on a 3D Enzyme/Carbon Nanotube Conductive Networks. Journal of Nanoscience and Nanotechnology, 17 (8). pp. 5896-5899. ISSN 1533-4880 (Print); 1533-4899 (Online). (Published)
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Abstract
We report on a novel 3-dimensional (3D) network of crosslinked Cytochrome C/Carbon Nanotube (CytC/CNT) on a thiol-modified gold surface which can establish direct electrical communication between the redox center of Cytochrome C and the electrode. Cyclic voltammograms (CVs) results showed a pair of well defined redox peaks for Cytochrome C, located at about −0.03 and +0.06 V, cathodic and anodic respectively. Additionally, the formal potential E0 of adsorbed Cyt c was found to be 15 mV, a value close to that of native Cyt c. Based on 3D Cytochrome c and carbon nanotube network, a sensitive superoxide radical biosensor has been proposed. The biosensor showed high sensitivity and lower detection limit of 0.3 �M of superoxide.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | 3D Crosslinked Networks, Cytochrome C, Superoxide, Biosensor |
| Subjects: | Q Science > QD Chemistry |
| Faculty/Division: | Faculty of Industrial Sciences And Technology |
| Depositing User: | Dr. A. K. M. Kafi |
| Date Deposited: | 10 Apr 2017 03:22 |
| Last Modified: | 26 Jul 2018 03:23 |
| URI: | http://umpir.ump.edu.my/id/eprint/15616 |
| Download Statistic: | View Download Statistics |
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