Sharifah Fathiyah, Sy Mohamad and Farhan, Mohd Said and Mimi Sakinah, A. M. and Shahril, Mohamad (2018) Factors influencing forward extraction of jacalin from aqueous phase into anionic reverse micelles. In: 4th International Conference Of Chemical Engineering And Industrial Biotechnology (ICCEIB 2018) , 1-2 August 2018 , Seri Pacific Hotel, Kuala Lumpur. pp. 51-52..
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Abstract
Background: This study deals with the extraction of jacalin from crude extract of jackfruit seed using anionic surfactant based reverse micellar system, dioctyl sulfosuccinate sodium salt (AOT) in isooctane. The effect of AOT concentration, NaCl concentration and aqueous phase pH on the forward extraction efficiency of jacalin was evaluated using one factor at a time experiments (OFAT). Results: AOT concentration, NaCl concentration and aqueous phase pH were found to have different effects on the removal of jacalin from the aqueous phase into the AOT reverse micelles. A maximum forward extraction efficiency of 78% was achieved after the mixture was stirred for 15 min using 10 mM AOT at pH 5 aqueous phase containing 0.1 M NaCl. Conclusion: The findings demonstrated AOT reverse micellar system as a promising and effective method to extract and purify jacalin from crude protein mixture.
| Item Type: | Conference or Workshop Item (Speech) |
|---|---|
| Uncontrolled Keywords: | Reverse micelles; Dioctyl sulfosuccinate sodium salt (AOT); Jacalin; Forward extraction efficiency (FEE) |
| Subjects: | T Technology > TP Chemical technology |
| Faculty/Division: | Faculty of Chemical & Natural Resources Engineering Faculty of Engineering Technology |
| Depositing User: | Pn. Hazlinda Abd Rahman |
| Date Deposited: | 20 Sep 2018 02:52 |
| Last Modified: | 02 Jan 2019 02:07 |
| URI: | http://umpir.ump.edu.my/id/eprint/21326 |
| Download Statistic: | View Download Statistics |
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