Nur 'Izzaty Hazera, Nazar (2016) Effect of operating conditions on characterization and stabilization of cyclodextrin glycosyltransferase (CGTASE). Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang.
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Abstract
Cyclodextrin glycosyltransferase (CGTase) is an enzyme that produces cyclodextrin (CD) from starch under catalytic reaction. It is an important industrial enzyme which mostly used in food and pharmaceutical industry. The purpose of this study is to determine the effect of operating conditions on characterization and stabilization of CGTase to improve the production of cyclodextrin (CD) in the future. Therefore, the effect of starch concentration and pH on the characterization of CGTase were studied. The effect of pH and temperature on stabilization of CGTase were investigated. The best starch concentration and pH on characterization of CGTase were 3% w.v and pH 6 respectively. The most stable conditions of CGTase were detected at pH 6-8 and temperature of 40-60˚C. By using these optimum conditions, the CD production may improve in the future which in turn may be beneficial to industrial uses.
| Item Type: | Undergraduates Project Papers |
|---|---|
| Additional Information: | Project Paper (Bachelors of Chemical Engineering) -- Universiti Malaysia Pahang – 2017, SV: DR ROHAIDA BT CHE MAN, NO. CD: 11152 |
| Uncontrolled Keywords: | Cyclodextrin glycosyltransferase; cyclodextrin (CD) |
| Subjects: | T Technology > TP Chemical technology |
| Faculty/Division: | Faculty of Chemical & Natural Resources Engineering |
| Depositing User: | Mrs. Sufarini Mohd Sudin |
| Date Deposited: | 25 Oct 2018 02:08 |
| Last Modified: | 03 Oct 2023 07:29 |
| URI: | http://umpir.ump.edu.my/id/eprint/22410 |
| Download Statistic: | View Download Statistics |
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