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Microbial lipid extraction from Lipomyces starkeyi using irreversible electroporation

Karim, Ahasanul and Yousuf, Abu and M. Amirul, Islam and H. Naif, Yasir and C. K. M., Faizal and Alam, Md. Zahangir and Pirozzi, Domenico (2018) Microbial lipid extraction from Lipomyces starkeyi using irreversible electroporation. Biotechnology Progress, 34 (4). pp. 838-845. ISSN 1520-6033

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Abstract

The aim of the study was to investigate the feasibility of using irreversible electroporation (EP) as a microbial cell disruption technique to extract intracellular lipid within short time and in an eco-friendly manner. An EP circuit was designed and fabricated to obtain 4 kV with frequency of 100 Hz of square waves. The yeast cells of Lipomyces starkeyi (L. starkeyi) were treated by EP for 2-10 min where the distance between electrodes was maintained at 2, 4, and 6 cm. Colony forming units (CFU) were counted to observe the cell viability under the high voltage electric field. The forces of the pulsing electric field caused significant damage to the cell wall of L. starkeyi and the disruption of microbial cells was visualized by field emission scanning electron microscopic (FESEM) image. After breaking the cell wall, lipid was extracted and measured to assess the efficiency of EP over other techniques. The extent of cell inactivation was up to 95% when the electrodes were placed at the distance of 2 cm, which provided high treatment intensity (36.7 kWh m−3). At this condition, maximum lipid (63 mg g−1) was extracted when the biomass was treated for 10 min. During the comparison, EP could extract 31.88% lipid while the amount was 11.89% for ultrasonic and 16.8% for Fenton's reagent. The results recommend that the EP is a promising technique for lowering the time and solvent usage for lipid extraction from microbial biomass.

Item Type: Article
Uncontrolled Keywords: cell disruption; electroporation; lipid composition; Lipomyces starkeyi
Subjects: T Technology > T Technology (General)
T Technology > TP Chemical technology
Faculty/Division: Faculty of Chemical & Natural Resources Engineering
Faculty of Engineering Technology
Depositing User: Mrs. Neng Sury Sulaiman
Date Deposited: 05 Dec 2018 01:33
Last Modified: 05 Dec 2018 01:33
URI: http://umpir.ump.edu.my/id/eprint/22932
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