Fractional factorial analysis for identifying significant factors in extraction of jacalin from jackfruit seeds using anionic reverse micellar system

Sharifah Fathiyah, Sy Mohamad and Farhan, Mohd Said and Mimi Sakinah, A. M. and Shahril, Mohamad and Wan Azizi, Wan Sulaiman Fractional factorial analysis for identifying significant factors in extraction of jacalin from jackfruit seeds using anionic reverse micellar system. In: International Conference on Chemical Sciences and Engineering: Advance and New Materials (ICCSE 2018) , 7 - 8 November 2018 , Ramada Plaza, Melaka. pp. 1-9.. (Submitted)

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Abstract

Reverse micellar extraction is emerging as one of the most reliable methods for extraction and purification of various proteins and enzymes. In this study, a fractional factorial design was used to identify the significant factors having the largest effects on the forward extraction process of jacalin using reverse micelles formed by an anionic surfactant, sodium bis(2-ethylhexyl) sulfosuccinate (AOT). Effect of five different factors, namely the aqueous phase pH, surfactant (AOT) concentration, salt (NaCl) concentration, contact time and the volume ratio of organic to aqueous phase on the forward extraction efficiency were investigated using the 25-1 fractional factorial design. The results showed that only the aqueous phase pH, NaCl concentration and concentration of AOT had significant effects on the forward extraction efficiency of jacalin into AOT reverse micelles. Results obtained indicated that the fractional factorial design was a useful method for screening many process factors with a minimum number of experiments.

Item Type:	Conference or Workshop Item (Speech)
Uncontrolled Keywords:	Forward extraction; Sodium bis(2-ethylhexyl) sulfosuccinate (AOT) micelles; Jacalin; Fractional factorial design
Subjects:	T Technology > T Technology (General) T Technology > TP Chemical technology
Faculty/Division:	Faculty of Chemical & Natural Resources Engineering Faculty of Engineering Technology
Depositing User:	Encik Shahril Mohamad
Date Deposited:	10 Jun 2019 07:44
Last Modified:	10 Jun 2019 07:44
URI:	http://umpir.ump.edu.my/id/eprint/24682
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