GPTMS-modified bredigite/PHBV nanofibrous bone scaffolds with enhanced mechanical and biological properties

Kouhi, Monireh and Jayarama Reddy, Venugopal and Ramakrishna, Seeram (2019) GPTMS-modified bredigite/PHBV nanofibrous bone scaffolds with enhanced mechanical and biological properties. Applied Biochemistry and Biotechnology, 188 (2). pp. 357-368. ISSN 0273-2289. (Published)

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Bioceramic nanoparticles with high specific surface area often tend to agglomerate in the polymer matrix, which results in undesirable mechanical properties of the composites and poor cell spreading and attachment. In the present work, bredigite (BR) nanoparticles were modified with an organosilane coupling agent, 3-glycidoxypropyltrimethoxysilane (GPTMS), to enhance its dispersibility in the polymer matrix. The polyhydroxybutyrate-co-hydroxyvaletare (PHBV) nanofibrous scaffolds containing either bredigite or GPTMS-modified bredigite (G-BR) nanoparticles were fabricated using electrospinning technique and characterized using scanning electron microscopy, transmission electron microscopy, and tensile strength. Results demonstrated that modification of bredigite was effective in enhancing nanoparticle dispersion in the PHBV matrix. PHBV/G-BR scaffold showed improved mechanical properties compared to PHBV and PHBV/BR, especially at the higher concentration of nanoparticles. In vitro bioactivity assay performed in the simulated body fluid (SBF) indicated that composite PHBV scaffolds were able to induce the formation of apatite deposits after incubation in SBF. From the results of in vitro biological assay, it is concluded that the synergetic effect of BR and GPTMS provided an enhanced hFob cells attachment and proliferation. The developed PHBV/G-BR nanofibrous scaffolds may be considered for application in bone tissue engineering.

Item Type: Article
Additional Information: Indexed by Scopus
Uncontrolled Keywords: Bredigite nanoparticles; PHBV; GPTMS; Electrospinning; Bone tissue engineering
Subjects: Q Science > QD Chemistry
R Medicine > R Medicine (General)
R Medicine > RD Surgery
T Technology > TP Chemical technology
Faculty/Division: Faculty of Industrial Sciences And Technology
Depositing User: Mrs Norsaini Abdul Samat
Date Deposited: 03 Jul 2019 08:34
Last Modified: 03 Jul 2019 08:34
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