S. D., Vijaya Kumar and Zainatul Bahiyah, Handani and Rohana, Abu (2019) Kinetic study of asymmetric synthesis of chiral amine with immobilized transaminase. In: Energy Security and Chemical Engineering Congress (ESCHE2019) , 17-19 July 2019 , Parkroyal Resort Penang, Malaysia. pp. 1-4.. (Unpublished)
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Abstract
Biocatalysis is a powerful tool for organic synthesis, especially for the synthesis of high value products such as chiral molecules and intermediates. Chiral amines can be synthesized by kinetic resolution of racemic amines or by the asymmetric synthesis from prochiral ketones. Kinetic parameter estimation of such biocatalytic reaction is useful to evaluate process and technology options. In this research, the Michaelis–Menten kinetic parameters from the asymmetric synthesis of (R)-1-phenylethylamine from acetophenone and alanine with immobilized ω-transaminase were estimated. The immobilized ω-transaminase was prepared by entrapment with diaion beads. The kinetic parameters such as, Michaelis-Menten constant (Km) and maximum rate of reaction (Vmax) were measured using initial rate experiments by varying the substrate acetophenone concentrations (2 mM to 10 mM) at 100 mM of amino donor. The kinetic parameters were then estimated by Lineweaver-burk analysis. The Vmax and Km was estimated at 6.33 mM/min and 0.382 mM, respectively.
| Item Type: | Conference or Workshop Item (Lecture) |
|---|---|
| Uncontrolled Keywords: | Organic Synthesis; Especially; Biocatalytic Reaction; Kinetic Parameters |
| Subjects: | T Technology > TP Chemical technology |
| Faculty/Division: | Faculty of Chemical & Natural Resources Engineering Institute of Postgraduate Studies |
| Depositing User: | Pn. Hazlinda Abd Rahman |
| Date Deposited: | 18 Mar 2020 02:29 |
| Last Modified: | 18 Mar 2020 02:29 |
| URI: | http://umpir.ump.edu.my/id/eprint/26906 |
| Download Statistic: | View Download Statistics |
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