Yeong, Yi Ling and Pang, Sook Fun and Afiqah, Yeop and M. M., Yusoff and Jolius, Gimbun (2019) Assessment of Maceration, Ultrasonic and Microwave Assisted Extraction for Total Phenolic Content, Total Flavonoid Content and Kaempferol Yield from Cassia alata via Microstructures Analysis. Materials Today: Proceedings, 19 (Part 4). 1273 - 1279. ISSN 2214-7853. (Published)
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Abstract
This paper presents the effect of different extraction methods on total phenolic content (TPC), total flavonoid content (TFC) and kaempferol yield from Cassia alata via microstructure analysis. TPC and TFC of C. alata were measured spectrophotometrically by using Folin Ciocalteu assay and aluminium chloride colorimetric assay, respectively. Identification and quantification of kaempferol were performed using an ultra-performance liquid chromatography coupled with photodiode array (UPLC-PDA). The results showed that microwave assisted extraction (MAE) has the highest TPC, TFC and kaempferol yield (37.92 ± 0.50 mg GAE/g DW, 135.18 ± 2.90 mg QE/g DW, 17.67 ± 1.06 mg KA/g DW), followed by ultrasonic assisted extraction (UAE) (26.91 ± 0.43 mg GAE/g DW, 86.69 ± 1.67 mg QE/g DW, 14.19 ± 0.43 mg KA/g DW) and maceration extraction (ME) (20.68 ± 1.63 mg GAE/g DW, 70.13 ± 4.43 mg QE/g DW, 12.01 ± 0.25 mg KA/g DW). The scanning electron microscopy analysis shows extensive contraction of the plant cell mesophyl and epidermis in the UAE and MAE compared to that of ME which yielded TPC and TFC about 24 to 93 higher than that of ME. The glandular trichomes completely dissapear from the MAE sample which explains the highest kaempferol yield obtained. The efficiency of polyphenols extraction relies on the disruption level of the C. alata leaves.
| Item Type: | Article |
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| Additional Information: | International Conference on Chemical Sciences and Engineering: Advance and New Materials, ICCSE 2018, 7 � 8 November 2018, Ramada Plaza Hotel Melaka Malaysia |
| Subjects: | T Technology > TP Chemical technology |
| Faculty/Division: | Centre of Excellence: Centre of Excellence for Advanced Research in Fluid Flow Faculty of Chemical & Natural Resources Engineering Faculty of Industrial Sciences And Technology Institute of Postgraduate Studies |
| Depositing User: | Prof. Dr. Jolius Gimbun |
| Date Deposited: | 27 Mar 2020 08:56 |
| Last Modified: | 27 Mar 2020 08:56 |
| URI: | http://umpir.ump.edu.my/id/eprint/27507 |
| Download Statistic: | View Download Statistics |
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