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Surface modification of polycaprolactone (PCL) microcarrier for performance improvement of human skin fibroblast cell culture

Nurhusna, Samsudin and Y. Z. H., Hashim and Mohd Azmir, Arifin and Maizirwan, Mel and Hamzah, Mohd Salleh and Sopyan, I. and Mariani H., Abdul Hamid (2018) Surface modification of polycaprolactone (PCL) microcarrier for performance improvement of human skin fibroblast cell culture. In: IOP Conference Series: Materials Science and Engineering, International Conference on Advances in Manufacturing and Materials Engineering (ICAMME 2017), 8-9 August 2017 , Kuala Lumpur, Malaysia. pp. 1-9., 290 (012016). ISSN 1757-8981 (Print), 1757-899X (Online)

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Abstract

Polycaprolactone (PCL) has many advantages for use in biomedical engineering field. In the present work PCL microcarriers of 150-200 μm were fabricated using oil-in-water (o/w) emulsification coupled with solvent evaporation method. The surface charge of PCL microcarrier was then been improved by using ultraviolet/ozone treatment to introduce oxygen functional group. Immobilisation of gelatin onto PCL microspheres using zero-length crosslinker provides a stable protein-support complex, with no diffusional barrier which is ideal for mass processing. The optimum concentration of carboxyl group (COOH) absorbed on the surface was 1495.9 nmol/g and the amount of gelatin immobilized was 1797.3 μg/g on UV/O3 treated microcarriers as compared to the untreated (320 μg/g) microcarriers. The absorption of functional oxygen groups on the surface and the immobilized gelatin was confirmed with Fourier Transformed Infrared spectroscopy and the enhancement of hydrophilicity of the surface was confirmed using water contact angle measurement which decreased (86.93° – 49.34°) after UV/O3 treatment and subsequently after immobilisation of gelatin. The attachment and growth kinetics for human skin fibroblast cell (HSFC) showed that adhesion occurred much more rapidly for gelatin immobilised surface as compared to untreated PCL and UV/O3 PCL microcarrier.

Item Type: Conference or Workshop Item (Lecture)
Additional Information: Indexed by Scopus
Uncontrolled Keywords: Fourier transformed infrared spectroscopy; Human skin fibroblast; Optimum concentration; Solvent evaporation method; UV/O3 treatment
Subjects: R Medicine > R Medicine (General)
R Medicine > RM Therapeutics. Pharmacology
T Technology > TP Chemical technology
Faculty/Division: Faculty of Engineering Technology
Depositing User: Mrs Norsaini Abdul Samat
Date Deposited: 13 Nov 2020 02:19
Last Modified: 13 Nov 2020 02:19
URI: http://umpir.ump.edu.my/id/eprint/29820
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