Rohaida, Che Man and Shalyda, Md Shaarani and Zatul Iffah, Mohd Arshad and Siti Kholijah, Abdul Mudalip and Siti Zubaidah, Sulaiman and Aizi Nor Mazila, Ramli (2020) Effect of reaction parameters on the production of cyclodextrin using cyclodextrin glucanotransferase from bacillus licheniformis. Journal of Chemical Engineering and Industrial Biotechnology (JCEIB), 6 (1). pp. 39-45. ISSN 0126-8139. (Published)
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Abstract
Cyclodextrin glucanotransferase (CGTase) is an industrial enzyme that converts starch into cyclodextrin (CD). CD has many potential applications in wide range of industries including food, cosmetic, pharmaceutical and plastic industries. Production of CD from CGTase is based on a few process parameters such as concentration of substrate, types of substrate, reaction time, pH and temperature. In this present study, the effect of types of substrate (corn, potato, wheat, rice and tapioca starch), concentration of substrate (1, 2, 3, 4, 5, 6, 7, 8 and 9%) and reaction time (1, 2, 3, 4 and 5 hr) on the CD production using commercial CGTase from Bacillus licheniformis were studied. Among the five types of substrate tested, the potato starch was the most suitable substrate for the production of CD with 10.24 mg/ml of CD. In addition, by using 6% (w/v) of potato starch, the production of CD was 15.16 mg/ml. The optimal reaction time was found to be 4 hr with 12.41 mg/ml of CD. Therefore, the best reaction parameters proved to be valuable for the highest CD production. Hence, CGTase from Bacillus licheniformis was demonstrated to produce high CD at optimum reaction conditions and it has potential to be used in large scale production for the industrial purposes.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Bacillus licheniformis, cyclodextrin glucanotransferase, cyclodextrin, types of substrate, concentration of substrate, reaction time |
| Subjects: | T Technology > TP Chemical technology |
| Faculty/Division: | Faculty of Industrial Sciences And Technology Institute of Postgraduate Studies College of Engineering Faculty of Chemical and Process Engineering Technology |
| Depositing User: | Noorul Farina Arifin |
| Date Deposited: | 10 Dec 2020 04:04 |
| Last Modified: | 10 Dec 2020 04:04 |
| URI: | http://umpir.ump.edu.my/id/eprint/30171 |
| Download Statistic: | View Download Statistics |
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