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Effect of reaction parameters on the production of cyclodextrin using cyclodextrin glucanotransferase from bacillus licheniformis

Rohaida, Che Man and Shalyda, Md Shaarani and Zatul Iffah, Mohd Arshad and Siti Kholijah, Abdul Mudalip and Siti Zubaidah, Sulaiman and Aizi Nor Mazila, Ramli (2020) Effect of reaction parameters on the production of cyclodextrin using cyclodextrin glucanotransferase from bacillus licheniformis. Journal of Chemical Engineering and Industrial Biotechnology (JCEIB), 6 (1). pp. 39-45. ISSN 0126-8139

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Cyclodextrin glucanotransferase (CGTase) is an industrial enzyme that converts starch into cyclodextrin (CD). CD has many potential applications in wide range of industries including food, cosmetic, pharmaceutical and plastic industries. Production of CD from CGTase is based on a few process parameters such as concentration of substrate, types of substrate, reaction time, pH and temperature. In this present study, the effect of types of substrate (corn, potato, wheat, rice and tapioca starch), concentration of substrate (1, 2, 3, 4, 5, 6, 7, 8 and 9%) and reaction time (1, 2, 3, 4 and 5 hr) on the CD production using commercial CGTase from Bacillus licheniformis were studied. Among the five types of substrate tested, the potato starch was the most suitable substrate for the production of CD with 10.24 mg/ml of CD. In addition, by using 6% (w/v) of potato starch, the production of CD was 15.16 mg/ml. The optimal reaction time was found to be 4 hr with 12.41 mg/ml of CD. Therefore, the best reaction parameters proved to be valuable for the highest CD production. Hence, CGTase from Bacillus licheniformis was demonstrated to produce high CD at optimum reaction conditions and it has potential to be used in large scale production for the industrial purposes.

Item Type: Article
Uncontrolled Keywords: Bacillus licheniformis, cyclodextrin glucanotransferase, cyclodextrin, types of substrate, concentration of substrate, reaction time
Subjects: T Technology > TP Chemical technology
Faculty/Division: Faculty of Industrial Sciences And Technology
Institute of Postgraduate Studies
College of Engineering
Faculty of Chemical and Process Engineering Technology
Depositing User: Noorul Farina Arifin
Date Deposited: 10 Dec 2020 04:04
Last Modified: 10 Dec 2020 04:04
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