Nur Husnina Iffah, Bakar and Thong, Chuan Lee and Mohd Hairul, Ab. Rahim and Raha, Ahmad Raus and Dayang Fredalina, Basri and Noor Suhana, Adzahar (2020) Real-time cytotoxicity assay of water extracts Hibiscus rosa-sinensis. Materials Science Forum, 981. pp. 265-270. ISSN 0255-5476. (Published)
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Abstract
A real-time impedance-based electrochemistry assay was used for assessing the cytotoxicity effect of water extract Hibiscus Rosa-Sinensis on Vero cell line. In this study, H. rosa-sinensis was extracted by using the water extraction with three different concentration which were 200, 100 and 50 µg/mL. The phytochemical compounds in the extract were analyzed by using the Gas Chromatography-Mass Spectrometry (GC-MS). The GC-MS result showed that the extract contains bioactive compounds such as n-hexadecanoic acid, pentadecanoic acid, phenol, 2,4-bis(1,1-dimethylethyl) and octadecanoic acid that have bioactive properties such as cytotoxic and antioxidant. The cytotoxic effect of the extract on normal cell line was assessed by using Real-Time Cell Analysis (RTCA) instrument. The result showed that there was no significant cytotoxicity effect against Vero cell lines. This result showed that the real-time impedance-based assay can be utilized to monitor changes of the cells and to determine the inhibitory concentration of the extract.
| Item Type: | Article |
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| Uncontrolled Keywords: | Cytotoxicity; Hibiscus; RTCA; Vero Cell |
| Subjects: | T Technology > T Technology (General) |
| Faculty/Division: | Faculty of Industrial Sciences And Technology Institute of Postgraduate Studies |
| Depositing User: | Pn. Hazlinda Abd Rahman |
| Date Deposited: | 08 Feb 2021 03:45 |
| Last Modified: | 08 Feb 2021 03:45 |
| URI: | http://umpir.ump.edu.my/id/eprint/30520 |
| Download Statistic: | View Download Statistics |
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