Abdi Wira, Septama and Ibrahim, Jantan and Panichayupakaranant, Pharkphoom and Mohd F. F., Mohd Aluwi and Eldiza Puji, Rahmi (2020) Immunosuppressive and antibacterial activities of dihydromorin and norartocarpetin isolated from artocarpus heterophyllus heartwoods. Asian Pacific Journal of Tropical Biomedicine, 10 (8). pp. 361-368. ISSN 2221-1691. (Published)
|
Pdf (Open access)
Immunosuppressive and antibacterial activities of dihydromorin and norartocarpetin.pdf Available under License Creative Commons Attribution Non-commercial Share Alike. Download (1MB) | Preview |
Abstract
Objective: To evaluate the immunosuppressive effect on human phagocytes and antibacterial activity of dihydromorin and norartocarpetin isolated from Artocarpus heterophyllus heartwoods. Methods: Dihydromorin and norartocarpetin were isolated from Artocarpus heterophyllus heartwoods. A modified Boyden chamber was used to determine the chemotactic activity of human phagocyte. The respiratory burst was evaluated by chemiluminescence assay. Myeloperoxidase (MPO) activity was quantified using a colorimetric assay. The broth microdilution method was performed to assess their antibacterial activity. Results: Dihydromorin exhibited potent inhibitory effect on the chemotactic activity of polymorphonuclear neutrophils (PMNs) with an IC50 value of 5.03 μg/mL. Dihydromorin also inhibited reactive oxygen species production of whole blood cells, PMNs, and monocytes with IC50 values of 7.88, 7.59 and 7.24 μg/mL, respectively. Interestingly, dihydromorin also strongly inhibited the MPO activity of PMNs with an IC50 value of 5.24 μg/mL, which was lower than indomethacin (24.6 μg/mL). Molecular docking of dihydromorin and crystal structure of MPO showed that dihydromorin had close interaction with key amino acid residues such as Arg239 and Gln91. Antibacterial activity assay showed that only dihydromorin had a strong effect against Streptococcus pyogenes with MIC and MBC values of 15.62 and 31.25 μg/mL, respectively. Conclusions: The results suggest that dihydromorin could be developed as an anti-inflammatory and antibacterial agent.
| Item Type: | Article |
|---|---|
| Additional Information: | Indexed by WOS |
| Uncontrolled Keywords: | Dihydromorin; Immunosuppressive; Antibacterial; Flavonoid; Myeloperoxidase; Artocarpus heterophyllus |
| Subjects: | T Technology > TP Chemical technology |
| Faculty/Division: | Faculty of Industrial Sciences And Technology |
| Depositing User: | Mrs Norsaini Abdul Samat |
| Date Deposited: | 30 Jun 2021 14:21 |
| Last Modified: | 30 Jun 2021 14:21 |
| URI: | http://umpir.ump.edu.my/id/eprint/30864 |
| Download Statistic: | View Download Statistics |
Actions (login required)
 |
View Item |