UMP Institutional Repository

Immunosuppressive and antibacterial activities of dihydromorin and norartocarpetin isolated from artocarpus heterophyllus heartwoods

Abdi Wira, Septama and Ibrahim, Jantan and Panichayupakaranant, Pharkphoom and Mohd F. F., Mohd Aluwi and Eldiza Puji, Rahmi (2020) Immunosuppressive and antibacterial activities of dihydromorin and norartocarpetin isolated from artocarpus heterophyllus heartwoods. Asian Pacific Journal of Tropical Biomedicine, 10 (8). pp. 361-368. ISSN 2221-1691

[img]
Preview
Pdf (Open access)
Immunosuppressive and antibacterial activities of dihydromorin and norartocarpetin.pdf
Available under License Creative Commons Attribution Non-commercial Share Alike.

Download (1MB) | Preview

Abstract

Objective: To evaluate the immunosuppressive effect on human phagocytes and antibacterial activity of dihydromorin and norartocarpetin isolated from Artocarpus heterophyllus heartwoods. Methods: Dihydromorin and norartocarpetin were isolated from Artocarpus heterophyllus heartwoods. A modified Boyden chamber was used to determine the chemotactic activity of human phagocyte. The respiratory burst was evaluated by chemiluminescence assay. Myeloperoxidase (MPO) activity was quantified using a colorimetric assay. The broth microdilution method was performed to assess their antibacterial activity. Results: Dihydromorin exhibited potent inhibitory effect on the chemotactic activity of polymorphonuclear neutrophils (PMNs) with an IC50 value of 5.03 μg/mL. Dihydromorin also inhibited reactive oxygen species production of whole blood cells, PMNs, and monocytes with IC50 values of 7.88, 7.59 and 7.24 μg/mL, respectively. Interestingly, dihydromorin also strongly inhibited the MPO activity of PMNs with an IC50 value of 5.24 μg/mL, which was lower than indomethacin (24.6 μg/mL). Molecular docking of dihydromorin and crystal structure of MPO showed that dihydromorin had close interaction with key amino acid residues such as Arg239 and Gln91. Antibacterial activity assay showed that only dihydromorin had a strong effect against Streptococcus pyogenes with MIC and MBC values of 15.62 and 31.25 μg/mL, respectively. Conclusions: The results suggest that dihydromorin could be developed as an anti-inflammatory and antibacterial agent.

Item Type: Article
Additional Information: Indexed by WOS
Uncontrolled Keywords: Dihydromorin; Immunosuppressive; Antibacterial; Flavonoid; Myeloperoxidase; Artocarpus heterophyllus
Subjects: T Technology > TP Chemical technology
Faculty/Division: Faculty of Industrial Sciences And Technology
Depositing User: Mrs Norsaini Abdul Samat
Date Deposited: 30 Jun 2021 14:21
Last Modified: 30 Jun 2021 14:21
URI: http://umpir.ump.edu.my/id/eprint/30864
Download Statistic: View Download Statistics

Actions (login required)

View Item View Item