Yeap, Swee Keong and Norlaily, Mohd Ali and Akhtar, Muhammad Nadeem and Nursyamirah, Abd Razak and Chong, Zhi Xiong and Ho, Wan Yong and Boo, Lily and Seema, Zareen and Tonni Agustiono, Kurniawan and Avtar, Ram and Ng, Stephanie Y. L. and Ong, Alan Han Kiat and Alitheen, Noorjahan Banu (2021) Induction of apoptosis and regulation of MicroRNA expression by (2E,6E)-2,6-bis-(4-hydroxy-3-methoxybenzylidene)-cyclohexanone (BHMC) treatment on MCF-7 breast cancer cells. Molecules, 26 (5). pp. 1-15. ISSN 1420-3049 (print); 1420-3049 (online). (Published)
|
Pdf (Open access)
Induction of apoptosis and regulation of microRNA expression.pdf Available under License Creative Commons Attribution. Download (21MB) | Preview |
Abstract
(2E,6E)-2,6-bis-(4-hydroxy-3-methoxybenzylidene)-cyclohexanone (BHMC) is a synthetic curcumin analogue, which has been reported to possess anti-tumor, anti-metastatic, and anti-invasion properties on estrogen receptor (ER) negative breast cancer cells in vitro and in vivo. However, the cytotoxic effects of BHMC on ER positive breast cancer cells were not widely reported. This study was aimed to investigate the cytotoxic potential of BHMC on MCF-7 cells using cell viability, cell cycle, and apoptotic assays. Besides, microarray and quantitative polymerase chain reaction (qPCR) were performed to identify the list of miRNAs and genes, which could be dysregulated following BHMC treatment. The current study discovered that BHMC exhibits selective cytotoxic effects on ER positive MCF-7 cells as compared to ER negative MDA-MB-231 cells and normal breast cells, MCF-10A. BHMC was shown to promote G2/M cell cycle arrest and apoptosis in MCF-7 cells. Microarray and qPCR analysis demonstrated that BHMC treatment would upregulate several miRNAs like miR-3195 and miR-30a-3p and downregulate miRNAs such as miR-6813-5p and miR-6132 in MCF-7 cells. Besides, BHMC administration was also found to downregulate few tumor-promoting genes like VEGF and SNAIL in MCF-7. In conclusion, BHMC induced apoptosis in the MCF-7 cells by altering the expressions of apoptotic-regulating miRNAs and associated genes.
| Item Type: | Article |
|---|---|
| Additional Information: | Indexed by Scopus |
| Uncontrolled Keywords: | Breast cancer; 2,6-bis-(4-hydroxy-3-methoxybenzylidene)-cyclohexanone (BHMC); MCF�7; Apoptosis; MiRNA |
| Subjects: | T Technology > TP Chemical technology |
| Faculty/Division: | Faculty of Industrial Sciences And Technology Institute of Postgraduate Studies |
| Depositing User: | Mrs Norsaini Abdul Samat |
| Date Deposited: | 20 Aug 2021 14:16 |
| Last Modified: | 20 Aug 2021 14:16 |
| URI: | http://umpir.ump.edu.my/id/eprint/31831 |
| Download Statistic: | View Download Statistics |
Actions (login required)
 |
View Item |