Rafiqul, Islam S. M. and Mimi Sakinah, Abdul Munaim and Zularisam, Abdul Wahid (2021) Improvement of enzymatic bioxylitol production from sawdust hemicellulose: optimization of parameters. Preparative Biochemistry & Biotechnology, 51 (10). pp. 1060-1070. ISSN 1532-2297. (Published)
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Abstract
Enzymatic production of bioxylitol from lignocellulosic biomass (LCB) provides a promising alternative to both chemical and fermentative routes. This study aimed to assess the impacts of catalytic variables on bioxylitol production from wood sawdust using xylose reductase (XR) enzyme and to optimize the bioprocess. Enzyme-based xylitol production was carried out in batch cultivation under various experimental conditions to obtain maximum xylitol yield and productivity. The response surface methodology (RSM) was followed to fine-tune the most significant variables such as reaction time, temperature, and pH, which influence the synthesis of bioxylitol from sawdust hydrolysate and to optimize them. The optimum time, temperature, and pH became were 12.25 h, 35 degrees C, and 6.5, respectively, with initial xylose 18.8 g/L, NADPH 2.83 g/L, XR 0.027 U/mg, and agitation 100 rpm. The maximum xylitol production was attained at 16.28 g/L with a yield and productivity of 86.6% (w/w) and 1.33 g/L center dot h, respectively. Optimization of catalytic parameters using sequential strategies resulted in 1.55-fold improvement in overall xylitol production. This study explores a novel strategy for using sawdust hemicellulose in bioxylitol production by enzyme technology.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Bioconversion; bioxylitol; hemicellulosic hydrolysate; optimization; xylose; xylose reductase |
| Subjects: | T Technology > TP Chemical technology |
| Faculty/Division: | College of Engineering Faculty of Chemical and Process Engineering Technology |
| Depositing User: | Prof. Dr. Mimi Sakinah Abd Munaim |
| Date Deposited: | 12 May 2022 07:13 |
| Last Modified: | 12 May 2022 07:13 |
| URI: | http://umpir.ump.edu.my/id/eprint/34063 |
| Download Statistic: | View Download Statistics |
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