Rashid, Shah Samiur and Mustafa, Abu Hasnat and Mohd Hasbi, Ab. Rahim and Gunes, Burcu (2022) Magnetic nickel nanostructure as cellulase immobilization surface for the hydrolysis of lignocellulosic biomass. International Journal of Biological Macromolecules, 209. 1048 -1053. ISSN 0141-8130. (Published)
![[img]](http://umpir.ump.edu.my/style/images/fileicons/other.png) |
Pdf
Magnetic nickel nanostructure as cellulase immobilization surface_FULL.pdf Restricted to Repository staff only Download (2MB) |
|
|
Pdf
Magnetic nickel nanostructure as cellulase immobilization surface .pdf Download (148kB) | Preview |
Abstract
In this research, a magnetic reusable nickel nanoparticle (NiNPs) supporting materials were prepared for cellulase enzyme immobilization. The immobilized cellulase showed high activity recovery, large & fast immobilization capacity and improved pH & temperature tolerance. The excellent stability and reusability enabled the immobilized cellulase to retain 84% of its initial activity after ten cycles. At 2 mg/mL enzyme concentration, highest 93% immobilization efficiency was achieved within two hours of immobilization. When the treatment temperature reached 40 °C and pH 5, the immobilized cellulase exhibited highest residual activity. The immobilized cellulase could be separated from the solution by a magnetic force. This study introduced a novel supporting material for cellulase immobilization, and the immobilized cellulase poses a great potential in the hydrolysis of lignocellulosic biomass which can used as an easily applicable and sustainable pre-treatment step for advanced biofuel production.
| Item Type: | Article |
|---|---|
| Additional Information: | Indexed by Scopus |
| Uncontrolled Keywords: | Nickel nanoparticles; Amino functionalization; Cellulase immobilization; Enzymatic hydrolysis; Enzyme reusability |
| Subjects: | T Technology > TP Chemical technology |
| Faculty/Division: | Faculty of Industrial Sciences And Technology Institute of Postgraduate Studies |
| Depositing User: | Mrs Norsaini Abdul Samat |
| Date Deposited: | 21 Jul 2022 05:01 |
| Last Modified: | 21 Jul 2022 05:01 |
| URI: | http://umpir.ump.edu.my/id/eprint/34754 |
| Download Statistic: | View Download Statistics |
Actions (login required)
 |
View Item |