Reddy, Chilakamarry Chaitanya and Mimi Sakinah, A. M. and Zularisam, A. W. and Khilji, Irshad Ahmad and Sirohi, Ranjna and Reddy, Venugopal Jayarama (2022) Bioconversion of glycerol waste to ethanol by Escherichia coli and optimisation of process parameters. Indian Journal of Experimental Biology (IJEB), 60 (9). pp. 681-688. (Published)
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Abstract
Biofuel is one of the best ways to reduce our dependence on fossil fuels. Ever since commercial biodiesel production began, waste glycerol, the biodiesel byproduct, has gained researchers’ interest, especially its recycling. Here, we explored using glycerol residue (carbon source) as a substrate in the fermentation process for ethanol production by Escherichia coli K12 in anaerobic conditions. The factors affecting the ethanol production was optimised by response surface methodology (RSM). Significant variables that impact the ethanol concentration were pH, temperature and the substrate, with a statistically significant effect (P <0.05) on ethanol formation. The significant factor was analyzed by the Box-Behnken design. The optimum conditions for bioethanol formation using glycerol as substrate was obtained at pH 7 and temperature 37°C. The ethanol productivity was 0.77 g/L/h. The ethanol concentration of 9.2 g/L achieved from glycerol residue was close to the theoretical value with the fermentation achieved at optimised terms.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Biodiesel; Box-Behnken design; Response surface methodology (RSM) |
| Subjects: | T Technology > TP Chemical technology |
| Faculty/Division: | Institute of Postgraduate Studies Faculty of Chemical and Process Engineering Technology Faculty of Civil Engineering Technology |
| Depositing User: | Noorul Farina Arifin |
| Date Deposited: | 06 Sep 2022 09:03 |
| Last Modified: | 06 Sep 2022 09:03 |
| URI: | http://umpir.ump.edu.my/id/eprint/35107 |
| Download Statistic: | View Download Statistics |
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