N. A. S. M., Rosdee and N., Masngut and S. M., Shaarani and S., Jamek and M. S. M., Sueb (2020) Enzymatic hydrolysis of lignocellulosic biomass from pineapple leaves by using endo-1,4xylanase : Effect of pH, temperature, enzyme loading and reaction time. In: IOP Conference Series: Materials Science and Engineering, Energy Security and Chemical Engineering Congress , 17-19 July 2019 , Kuala Lumpur, Malaysia. pp. 1-7., 736 (022095). ISSN 1757-8981
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Enzymatic hydrolysis of lignocellulosic biomass from pineapple leaves by using endo-1,4-xylanase_Effect of pH, temperature, enzyme loading.pdf Available under License Creative Commons Attribution. Download (548kB) | Preview |
Abstract
Pineapple leaves; is one of lignocellulosic materials that composed of about 80% of carbohydrate containing lignin, cellulose and hemicellulose This waste still retains a considerable amount of soluble sugars that can be transformed into valuable fermentable sugars. This research was conducted to study the effect of different pH, temperature, enzyme loading and reaction time in order to identify the best working conditions to produce the highest yield of sugar. Prior to enzymatic hydrolysis, the pineapple leaves were subjected to hydrothermal pretreatment in order to obtain the hydrolysate liquid. Enzymatic hydrolysis was then carried out by using endo-1,4-xylanase and the reducing sugar was analyzed by using dinitrosalicylic (DNS) method. The result has shown that the highest reducing sugar concentration of 70.9 mg/L was obtained both at pH 5.5 and 50 °C. In addition, the best enzyme loading and reaction time were recorded at 0.5% (w/v) and 45 min that resulted 60.1 mg/L and 72. 0 mg/L respectively.
| Item Type: | Conference or Workshop Item (Lecture) |
|---|---|
| Additional Information: | Indexed by Scopus |
| Uncontrolled Keywords: | Enzymatic hydrolysis of lignocellulosic; Biomass from pineapple leaves |
| Subjects: | Q Science > Q Science (General) Q Science > QD Chemistry T Technology > T Technology (General) T Technology > TA Engineering (General). Civil engineering (General) T Technology > TP Chemical technology |
| Faculty/Division: | Faculty of Industrial Sciences And Technology Institute of Postgraduate Studies Faculty of Chemical and Process Engineering Technology |
| Depositing User: | Mr Muhamad Firdaus Janih@Jaini |
| Date Deposited: | 28 Dec 2022 04:53 |
| Last Modified: | 28 Dec 2022 04:53 |
| URI: | http://umpir.ump.edu.my/id/eprint/35975 |
| Download Statistic: | View Download Statistics |
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