Noorhafiza Diyana, Yahya and Zatul Iffah, Mohd Arshad and Siti Kholijah, Abdul Mudalip and Siti Zubaidah, Sulaiman and Rohaida, Che Man and Shalyda, Md Shaarani @ Md Nawi and Mohd Hairul, Ab. Rahim (2020) Screening of extraction buffer concentration for protease extraction from the leaves of Syzygium polyanthum using full factorial design. In: IOP Conference Series: Materials Science and Engineering, Energy Security and Chemical Engineering Congress , 17-19 July 2019 , Kuala Lumpur, Malaysia. pp. 1-10., 736 (022082). ISSN 1757-8981
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Screening of extraction buffer concentration for protease extraction from the leaves of Syzygium polyanthum using full factorial design.pdf Available under License Creative Commons Attribution. Download (915kB) | Preview |
Abstract
This study aimed to identify the factors that significantly affect the extraction of protease from the leaves of Syzygium polyanthum. The extraction of protease from the leaves of Syzigium polyanthum was studied at different concentration of substances in the extraction buffer. A 24 full factorial experimental design was performed. The effect of four independent operating factors including; A, the concentration of potassium phosphate (KPO4); B, the concentration of Triton X-100; C, the concentration of glycerol; and D, the concentration of dithiotreitol (DTT) on the protease activity were evaluated statistically. All regression models gave a good fit to the experimental data (R2 > 99%). The maximum protease activity was detected when the extraction was performed using 3.0 M KPO4, 1% Triton X-100, 80% glycerol and 1.5 M DTT. The results revealed that the concentration of KPO4, DTT, and their interaction were statistically significant to affect the protease activity. Thus, the results obtained from this study indicate that full factorial design is useful to screen the significant factors and minimise the number of experiments.
| Item Type: | Conference or Workshop Item (Lecture) |
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| Additional Information: | Indexed by Scopus |
| Uncontrolled Keywords: | Enzymes; Tributyrin; 4-Nitrophenyl laurate |
| Subjects: | Q Science > QD Chemistry T Technology > T Technology (General) T Technology > TA Engineering (General). Civil engineering (General) T Technology > TP Chemical technology |
| Faculty/Division: | Faculty of Chemical & Natural Resources Engineering Institute of Postgraduate Studies College of Engineering Faculty of Chemical and Process Engineering Technology Faculty of Industrial Sciences And Technology |
| Depositing User: | Mr Muhamad Firdaus Janih@Jaini |
| Date Deposited: | 28 Dec 2022 01:38 |
| Last Modified: | 28 Dec 2022 01:38 |
| URI: | http://umpir.ump.edu.my/id/eprint/36011 |
| Download Statistic: | View Download Statistics |
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