Sequential adaptation to serum-free medium for Vero cells cultivation on ultraviolet/ozone (UVO) treated microcarrier

Swan, Sia Yiik and Nurul Izzati, Azahar and Lansayan, Jalviana and Wong, Judy and Mohd Azmir, Arifin (2022) Sequential adaptation to serum-free medium for Vero cells cultivation on ultraviolet/ozone (UVO) treated microcarrier. In: The 6th National Conference for Postgraduate Research (NCON-PGR 2022) , 15 November 2022 , Virtual Conference, Universiti Malaysia Pahang, Malaysia. p. 94..

Sequential adaptation to serum-free medium for vero cells cultivation on ultraviolet_ozone (uvo) treated microcarrier.pdf

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Vero cells are continuous mammalian cell lines that are commonly used in research, viral vaccines production, recombinant protein production, and many others. The main objective of this study was to adapt the Vero cells in serum-free medium (SFM) from serum supplemented medium (SSM) prior to culture upscale from T-flask to spinner vessel using ultraviolet/ozone (UVO) treated polystyrene (PS) and polycaprolactone (PCL) microcarriers. The number of cells was counted using hemocytometer and microscope. From the results, it was observed that the maximum cell concentration of SFM adapted cells is higher than the cells cultured in SSM along with shorter doubling time. Culture of Vero cells in spinner vessel showed lower maximum cell concentrations compared to in T-flask due to several factors such as cell inoculum size, hydrophilicity of microcarriers and elevated temperature during incubation.

Item Type: Conference or Workshop Item (Lecture)
Uncontrolled Keywords: Vero cell; Serum-free medium; Polystyrene microcarrier; Polycaprolactone microcarrier.
Subjects: Q Science > QD Chemistry
T Technology > T Technology (General)
T Technology > TA Engineering (General). Civil engineering (General)
T Technology > TP Chemical technology
Faculty/Division: Institute of Postgraduate Studies
College of Engineering
Faculty of Chemical and Process Engineering Technology
Depositing User: Mr Muhamad Firdaus Janih@Jaini
Date Deposited: 20 Feb 2023 04:12
Last Modified: 10 Jan 2024 08:05
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