Production and Optimization of L-asparaginase by Bacillus sp. KK2S4 From Corn Cob

Makky, Essam A. and Jee, Jian Ong and Karim, Md. Rezaul and Lee, Chin Mei (2013) Production and Optimization of L-asparaginase by Bacillus sp. KK2S4 From Corn Cob. African Journal of Biotechnology, 12 (19). pp. 2654-2658. ISSN 1684-5315. (Published)

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Abstract

L-Asparaginase (L-asparagine amido hydrolase, E.C.3.5.1.1) is an important enzyme which has anti-tumor properties. This paper describes the production and optimization of L-asparaginase by Bacillus sp. KK2S4 utilization of ground corn cob waste as substrate to reduce and manage the waste in our environment. Thirty-nine (39) bacterial isolates were screened for their L-asparaginase productivity and protein content. Only Bacillus sp KK2S4 was identified before and selected as the most potent isolate. Modified M9 (MM9) minimal complete medium (glucose free) was used as production media for enzyme production. The highest enzyme production was detected at pH 5.0 in presence of lactose and NaNO3 as the best carbon and nitrogen sources with high activity increasing percentage of 1058.9, 377.3 and 566.7%, respectively.

Item Type:	Article
Additional Information:	DOI: 10.5897/AJB2013.12231 IF: 0.57 Please Click Official URL
Uncontrolled Keywords:	L-Asparaginase production; Corn cob waste; Enzyme optimization; Anti-tumor agent
Subjects:	T Technology > TP Chemical technology
Faculty/Division:	Faculty of Industrial Sciences And Technology
Depositing User:	Noorul Farina Arifin
Date Deposited:	05 Jun 2013 01:44
Last Modified:	14 Aug 2017 04:15
URI:	http://umpir.ump.edu.my/id/eprint/3780
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