Polymer-salt interaction

Jun Wei Roy, Chong and Zatul Iffah, Mohd Arshad and Kit Wayne, Chew and Pau Loke, Show (2023) Polymer-salt interaction. In: Principles of Multiple-Liquid Separation Systems Interaction, Application and Advancement. Elsevier, pp. 21-44. ISBN 9780323917285

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Abstract

Up to this moment, an uprising demand in downstream processing by incorporate innovative, effective, and reliable extraction methods as plenty effort and progression in the upstream production have led to advancement in the biomanufacturing industry. Previously, deployment of conventional downstream technologies has been a hindrance due to high cost, time-consuming, low yield, detrimental, noneco-friendly, lack of simplicity, and difficulties in scaling-up. A new emerging liquid–liquid extraction technology by means the polymer–salt aqueous two phases system for the recovery of biomolecules such as proteins, DNA, and nucleic acids, virus-like particles, and drug residues in food and water. Moreover, the partition coefficient of polyethylene glycol (PEG) 6000-phosphate aqueous two-phase system (ATPS) increased by 62-fold by adding NaCl which results in the recovery of proteins from 90% to 95% in a single purification step. Besides, plasmid DNA successfully recovered by 67% in the PEG-rich phase and managed to partition the contaminants toward the salt-rich phase. The total recovery of 90% from the separation of adenoviral vectors in HEK 293 cells using PEG-ammonium sulfate ATPS. In addition, the recovery of drug residues from food and water corresponds to recovery rate of 96%–100% which is safe for human consumption. This review discusses the following basic mechanism and working principles of ATPS. Factors affecting the partitioning behavior of biomolecules for instance, PEG molecular weight, PEG concentration, system pH, effect of temperature, hydrophobicity, and addition of salt are presented. Future consideration and modification to allow further improvement toward reliable large-scale extraction and purification of biomolecules.

Item Type:	Book Chapter
Additional Information:	Indexed by Scopus
Uncontrolled Keywords:	Biomolecules; Partitioning; Polymer–salt aqueous two-phase system; Purification; Recovery
Subjects:	T Technology > TP Chemical technology
Faculty/Division:	Faculty of Chemical & Natural Resources Engineering
Depositing User:	Miss Amelia Binti Hasan
Date Deposited:	15 Aug 2023 04:05
Last Modified:	15 Aug 2023 04:05
URI:	http://umpir.ump.edu.my/id/eprint/38390
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