Mior Ahmad Khushairi, Mohd Zahari (2013) Scaling up of poly (3-hydroxybutyrate) production from oil palm frond juice by Cupriavidus necator (CCUG52238T). Research Journal of Chemistry and Environment, 17 (7 ). pp. 18-24. ISSN 0972-0626 (Print), 2278-4527 (online). (Published)
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Abstract
It has been reported that oil palm frond juice (OPF) can be used as the sole renewable, alternative and cheap carbon source for the production of poly(3- hydroxybutyrate), P(3HB) at lab scale study. In order to further investigate the potential of OPF juice for P(3HB) production at larger scale, we scaled up the fermentation process from 500 ml shake flask to 20 L bioreactor based on constant volumetric mass transfer coefficient (kLa) of oxygen at both scale. It was interesting to note that the P(3HB) content obtained in this study was almost similar as reported in the previous study both in shake flask and 2 L bioreactor. The P(3HB) content for shake flask fermentation was 45 wt.%, while 44 wt.% and 42 wt.% in 2 L and 20 L bioreactor respectively. Based on the current finding, it can be concluded that the scaling up method based on constant kLa was successful due to the almost similar P(3HB) content obtained in shake flask and bioreactor scale. Overall, it can be postulated that OPF juice can be successfully used as a non-food fermentation feedstock for the production of P(3HB) both in small and large scale fermentation.
| Item Type: | Article |
|---|---|
| Additional Information: | Impact Factor : 0.636 Paper presented at 6th International Congress of Chemistry and Environment 2013, 8-10 July, Antwerp, Belgium |
| Uncontrolled Keywords: | Oil palm frond; Renewable sugars; Non-food fermentation feedstock; Poly(3-hydroxybutyrate); Volumetric mass transfer coefficient |
| Subjects: | T Technology > TP Chemical technology |
| Faculty/Division: | Faculty of Chemical & Natural Resources Engineering |
| Depositing User: | Noorul Farina Arifin |
| Date Deposited: | 21 Aug 2013 08:02 |
| Last Modified: | 30 Aug 2017 07:28 |
| URI: | http://umpir.ump.edu.my/id/eprint/3884 |
| Download Statistic: | View Download Statistics |
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