Extraction and characterization of biological phytoconstituents of commiphora gileadensis leaves using soxhlet method

Aiman A., Mokaizh and Nour, Abdurahman Hamid and Alazaiza, M. Y. D. and Mustafa, Sara Elkheir and Omer, Manal Suliman and Nassani, Dia Eddin (2024) Extraction and characterization of biological phytoconstituents of commiphora gileadensis leaves using soxhlet method. Processes, 12 (1567). pp. 1-11. ISSN 2227-9717. (Published)

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Abstract

Commiphora gileadensis is a medicinal plant with a wide range of biological characteristics. Many medical diseases can be treated using the leaves of C. gileadensis, including bacterial infections, inflammatory illnesses, and wounds. As a result, the Soxhlet extraction method was used to extract the phenolic components and measure the recovery yields from C. gileadensis leaf. The impacts of the Soxhlet extraction parameters (extraction time 30–150 min, sample/solvent ratio 1:20–1:40 g/mL, and concentration of ethanol solvent 20–100% v/v) on the total flavonoid content (TFC), total phenolic content (TPC), and extraction yield were investigated using the one-factor-at-a-time (OFAT) technique. Fourier transform infrared spectroscopy (FTIR) and gas chromatography–mass spectroscopy (GC–MS) analyses have been employed to evaluate the extracts for the presence of various phytochemicals. According to the results, the C. gileadensis leafs ethanolic extract extracted via the Soxhlet process achieved the maximum yields at 90 min of extraction time, a feed/solvent ratio of 1:30 g/mL, and a 40% v/v ethanol concentration. These yields were: extraction yield = 23.20 ± 0.10% w/w, TPC = 59.93 ± 1.33 mg GAE/g d.w., and TFC = 19.65 ± 1.77 mg QE/g d.w.. Further, a total of 20 phenolic components with excellent antioxidant characteristics were found in the leaf extract of C. gileadensis extracted via the Soxhlet method.

Item Type: Article
Uncontrolled Keywords: extraction process; total phenolic content; C. gileadensis leaf; total flavonoid content
Subjects: T Technology > TP Chemical technology
Faculty/Division: Institute of Postgraduate Studies
Faculty of Chemical and Process Engineering Technology
Depositing User: Miss Amelia Binti Hasan
Date Deposited: 15 Aug 2024 05:03
Last Modified: 15 Aug 2024 05:03
URI: http://umpir.ump.edu.my/id/eprint/42371
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