Factorial analysis of xylanase and cellulase production from pineapple peel waste

Sivanesan, Pavethra and Zatul Iffah, Mohd Arshad and Jun Haslinda, Shariffuddin and Nasratun, Masngut and Norazwina, Zainol and Shalyda, Md Shaarani (2022) Factorial analysis of xylanase and cellulase production from pineapple peel waste. In: Materials Science Forum. Trans Tech Publications Ltd, Switzerland, pp. 241-253. ISBN 1662-9752

Preview	Pdf Factorial Analysis of Xylanase and Cellulase Production from Pineapple Peel Waste - intro.pdf Download (265kB) | Preview
	Pdf Factorial Analysis of Xylanase and Cellulase Production from Pineapple Peel Waste.pdf Restricted to Repository staff only Download (804kB) | Request a copy

Abstract

Pineapple wastes (skin, core and crown) are mainly composed of carbohydrates (cellulose, hemicellulose, and lignin). Non-starch polysaccharides (NSP) in feed are indigestible by the endogenous enzymes in poultry. Thus, exogenous enzymes (xylanase and cellulase) are required to overcome this problem. Due to high fiber content, pineapple wastes are unsuitable for animal feed. However, the fermented waste juice could be used to produce enzymes. The objective of this study is to produce xylanase and cellulase from the fermentation of pineapple waste using Lactobacillus casei (L. casei) bacteria inoculated from probiotic drink. The fermentation was performed using different screening parameters (incubation time, temperature, pH value and substrate concentration) according to Two-Level Full Factorial Design (FFD) by Design Expert. From this study, the incubation temperature and substrate concentration had the highest influence on the xylanase activity (39.82 U/mL) while the fermentation time and substrate concentration mostly affected the cellulase activity (8.05 U/mL). Meanwhile, the pH had the least influence on both enzyme activities. The pineapple waste at its best fermentation parameters not only offers an economical way of high enzyme production but also alleviates the agricultural waste disposal issue. Further optimization of the pineapple waste fermentation parameters is required though to maximize enzyme production.

Item Type:	Book Chapter
Additional Information:	Indexde by Scopus
Uncontrolled Keywords:	pineapple peel waste; xylanase; cellulase; fermentation
Subjects:	T Technology > TP Chemical technology
Faculty/Division:	Institute of Postgraduate Studies College of Engineering Faculty of Chemical and Process Engineering Technology
Depositing User:	Miss Amelia Binti Hasan
Date Deposited:	02 Oct 2024 04:56
Last Modified:	02 Oct 2024 04:56
URI:	http://umpir.ump.edu.my/id/eprint/42718
Download Statistic:	View Download Statistics

Actions (login required)

View Item